Transcriptomics
Getting started guides
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A guide to transcriptomics with Oxford Nanopore
This guide introduces cDNA and direct RNA Oxford Nanopore sequencing, for ultra-rich transcriptomic data without compromise.
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A guide to single-cell transcriptomics with Oxford Nanopore
This guide introduces the single-cell RNA nanopore sequencing workflow, for ultra-rich data without compromise.
Workflow overviews
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Detecting isoforms and RNA modifications with PCR-free, direct RNA nanopore sequencing
This end-to-end workflow provides a simple method to characterise RNA modifications from a human blood research sample using direct RNA sequencing.
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Sequencing full-length transcripts for isoform-level expression analysis
This end-to-end workflow provides a flexible method to sequence full-length transcripts for isoform-level expression analysis.
Most viewed
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cDNA-PCR Sequencing Kits
In this flyer, discover how you can sequence full-length transcripts and annotate the transcriptome with confidence.
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Direct RNA Sequencing Kits
In this flyer, discover how you can sequence RNA in its native form. Plus, learn how the new Direct RNA Barcoding Kit unlocks multiplexed sequencing of up to 24 samples in a single run.
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Multiomic nanopore sequencing: revolutionising human disease research
Find out how multiomic nanopore sequencing can accelerate your disease research using a single platform.
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Long cDNA sequencing reads enable transcriptome analysis at isoform resolution
In this application note, we evaluated the updated Oxford Nanopore cDNA-PCR sequencing protocol updated protocol and compared its performance with that of the previous version of the protocol, Illumina short-read RNA-seq, and Pacific Biosciences Kinnex.
Featured resources
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Unlocking RNA biology with full-length reads
Discover how nanopore sequencing uniquely captures full-length transcripts for isoform-level transcriptome characterisation.
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Large-cohort cDNA sequencing advances multiomic insights into neurodegenerative disease
In this case study, discover how Kimberley Billingsley and her colleagues at the National Institutes of Health are using Oxford Nanopore large-cohort cDNA sequencing to research the human brain transcriptome, enhancing understanding of the drivers of neurodegenerative diseases.
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How do I analyse my Oxford Nanopore sequencing data without specialist training?
In this beginner-friendly masterclass, discover how to analyse your Oxford Nanopore sequencing data. Find out how basecalling works, the common file formats you’ll encounter, and how to use MinKNOW to set up and monitor your sequencing run. Then, discover how to analyse your data with EPI2ME — no pr
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Which library prep workflow is right for my experiment?
Whether you’re sequencing DNA, RNA, or cDNA, a single sample or many in multiplex, there’s an Oxford Nanopore library prep kit for you. In this masterclass, discover how to choose the right one for your workflow. In this masterclass, discover: • The range of Oxford Nanopore library prep solutions av
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How do I extract high-quality DNA and RNA for my Oxford Nanopore sequencing experiment?
Great sequencing results start with high-quality DNA and RNA extraction. In this masterclass, find out how to achieve just that — from choosing the right method for you to performing size selection, fragmentation, and sample handling. In this masterclass, discover: • How to optimise extraction for y
Protocols
cDNA-PCR Sequencing V14 (SQK-PCS114)
cDNA-PCR Sequencing V14 (SQK-PCS114)
cDNA-PCR 测序 V14 - 条形码文库构建（SQK-PCB114.24）
本文档介绍了一种最便捷高效的全长 cDNA 测序流程。
该流程具有以下特点：
- 实现最高测序产量
- 产量优于传统的 cDNA 合成方法
- 可检测剪接变异及融合转录本
- 支持多达24个不同样本的混合测序
- 仅兼容 R10.4.1 测序芯片 仅供研究使用
Analysis workflows
Latest research
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Analytical assessment of sgRNA impurities and their impact on functional performance
Publication: Analytical assessment of sgRNA impurities and their impact on functional performance
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Integrated long-read transcriptomic profiling of peripheral blood from ankylosing spondylitis patients identifies regulatory shifts and core genes associated with programmed cell death
Publication: Integrated long-read transcriptomic profiling of peripheral blood from ankylosing spondylitis patients identifies regulatory shifts and core genes associated with programmed cell death
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Female-specific m6A remodelling in the liver correlates with post transcriptional metabolic adaptation to high fat diet
Publication: Female-specific m6A remodelling in the liver correlates with post transcriptional metabolic adaptation to high fat diet
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Decoding the human PBMC isonome: isoform-level resolution with single-cell long-read transcriptomics
Publication: Decoding the human PBMC isonome: isoform-level resolution with single-cell long-read transcriptomics
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EPI2ME end-to-end workflows for biopharma quality control of plasmids and mRNA
Sample preparation, bespoke basecalling and medaka models, the Q-Line GridION device, and EPI2ME analysis workflows are revolutionising quality control in biopharma.
