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Getting started guides

Preview of the microbial getting sequencing getting started guideGetting started guide

A guide to microbial sequencing with Oxford Nanopore

A guide to get started with sequencing microbial samples with Oxford Nanopore.


Most viewed

Preview for the microbial sequencing brochureBrochure

Oxford Nanopore sequencing solutions for microbiology and infectious disease research

In this brochure, discover Oxford Nanopore sequencing techniques that can reveal multidimensional insights for your microbial research applications.
Plot showing the relationship between depth of coverage of nanopore reads and the accuracy of consensus sequences generated by EPI2ME wf-amplicon versus Sanger sequencing.Application note

A successor to Sanger: accurate, full-length, and haplotype-resolved amplicon sequencing with Oxford Nanopore

In this application note, we evaluate Oxford Nanopore sequencing for amplicon analysis using matched datasets generated with both Oxford Nanopore and Sanger sequencing.
Image of metagenomics application noteApplication note

Oxford Nanopore sequencing provides superior metagenome-assembled genome recovery and strain-level resolution from a complex microbiome

In this application note, we demonstrate the capabilities of Oxford Nanopore metagenomics by sequencing and analysing the well-characterised ZymoBIOMICS Fecal Reference.
Application note: bacterial isolate sequencing — 2 pagesApplication note

Oxford Nanopore whole-genome sequencing of foodborne pathogens

In this application note, we demonstrate the suitability of Oxford Nanopore sequencing as a standalone platform for foodborne pathogen whole-genome sequencing.
GridION flyer cover imageBrochure

GridION: one sequencer for many

Made with multiple projects and teams in mind, GridION gives you the freedom to start, stop, and scale your sequencing experiments without impacting others. Meanwhile, onboard compute keeps your real-time data optimised. Discover more.

Workflow overviews

Preview of the NO-MISS workflow overviewWorkflow overview

Nanopore-only microbial isolate sequencing solution (NO-MISS): flexible, rapid bacterial and fungal isolate whole-genome sequencing

This end-to-end workflow provides a rapid solution for whole-genome Oxford Nanopore sequencing of bacterial and fungal isolates.
Preview of the microbial amplicon barcoding workflow.Workflow overview

Streamlined identification of bacteria, archaea, and fungi with Oxford Nanopore microbial amplicon barcoding

This end-to-end workflow introduces how to sequence the full length 16S gene and ITS region with Oxford Nanopore technology.
Workflow: plasmid sequencing — 2 pagesWorkflow overview

Rapid and complete plasmid characterisation with Oxford Nanopore sequencing

This end-to-end workflow is a rapid method for complete, high-quality whole-plasmid characterisation.
Microbiology stillWorkflow overview

Rapid viral sequencing for genomic surveillance of mpox using metagenomic or targeted approaches

This end-to-end workflow provides guidance for both metagenomic and targeted viral genome sequencing to deliver rapid and actionable results.
Image preview of the pathogen metagenomics workflow overview.Workflow overview

Rapid identification of respiratory pathogens with Oxford Nanopore metagenomics

This end-to-end workflow introduces how to rapidly identify bacterial, fungal, and viral pathogens from respiratory research samples using metagenomic Oxford Nanopore sequencing on a MinION or GridION.

Visualisation of a double-stranded DNA molecule in dark blueCase study

One size fits all: development of a simple workflow to characterise any viral pathogen

In the case study, find out how nanopore sequencing can be used to sequence any viral pathogen in low-resource settings.
Adela_Alcolea-Medina_and_Luke_Blagdon SnellEvent video

Embedding clinical metagenomics into NHS diagnostic pathways | LC26

Clinical metagenomics is a rapidly advancing field in infectious disease diagnostics, using next-generation sequencing to identify pathogens directly from clinical samples without prior assumptions. In this presentation, Dr Adela Alcolea-Medina will describe the development, validation, and national
Real-time targeted sequencingEvent video

How do I perform real-time targeted Oxford Nanopore sequencing?

What if targeted sequencing took place during sequencing itself, without the need for special library prep, PCR, or probes? In this demo, discover Adaptive Sampling: a unique, real-time targeted enrichment/depletion method that achieves just that.
Microbiology MetagenomicsCase study

Pan-microbial pathogen detection in hours using metagenomic sequencing

In this case study, discover how the UK NHS Respiratory Metagenomics Network have developed a rapid Oxford Nanopore sequencing workflow that can detect the bacteria, viruses, fungi, and parasitic pathogens underlying respiratory infections in a matter of hours.
Which_library_prep_workflow_is_right_for_my_experimentEvent video

Which library prep workflow is right for my experiment?

Whether you’re sequencing DNA, RNA, or cDNA, a single sample or many in multiplex, there’s an Oxford Nanopore library prep kit for you. In this masterclass, discover how to choose the right one for your workflow.

Protocols

SQK-RBK110.96 gDNA workflow v1Workflow

快速DNA测序V14 - 条形码(SQK-RBK114.24 或 SQK-RBK114.96)

本实验指南: - 使用基因组DNA - 支持为1-96个样本混样测序 - 建库用时仅需约60分钟 - 产出量高 - 包含片段化步骤 - 与R10.4.1 测序芯片兼容 仅供研究使用

基因组DNA连接法测序 - 免扩增条形码测序试剂盒-24(SQK-NBD114.24)

本文档描述了基因组 DNA 的免扩增条形码建库流程。 实验指南: - 使用免扩增条形码测序试剂盒-24 V14 (SQK-NBD114.24) - 无需PCR扩增 - 支持多达24种条形码 - 可分析天然DNA - 与R10.4.1 测序芯片兼容 仅供研究使用

基因组DNA连接法测序 - 免扩增条形码测序试剂盒-96(SQK-NBD114.96)

本文档描述了基因组 DNA 的免扩增条形码建库流程。 实验指南: - 使用免扩增条形码测序试剂盒-96 V14 (SQK-NBD114.96) - 无需PCR扩增 - 支持多达96种条形码 - 可分析天然DNA - 与R10.4.1 测序芯片兼容 仅供研究使用
GridION DxWorkflow

SARS-CoV-2 virus PCR tiling and sequencing from RNA using SQK-RBK114.96

This protocol is for the extraction and sequencing of ultra-high molecular weight (uHMW) genomic DNA.

Influenza virus sequencing from RNA using SQK-NBD114 (.24 or .96)

This document describes the sequencing of the influenza virus. For Research Use Only

Analysis workflows

wf-tb-amr

wf-tb-amr is a workflow for determining the antibiotic resistance of Mycobacterium tuberculosis targeted sequencing samples.

wf-mpx

This workflow provides a simple way to analyse mpox sequencing data; taking raw Oxford Nanopore Technologies reads and creating a draft consensus and assembly.

wf-metagenomics

This workflow can be used for the taxonomic classification of metagenomic sequencing data.

wf-flu

This analysis workflow can be used with Oxford Nanopore Technologies sequencing data from amplified segments of the Influenza Type A and Type B genomes, to determine the most likely strain of Influenza to which the sequenced sample belongs.

wf-bacterial-genomes

This workflow is primarily used to assemble genomes from bacterial reads and provide information on features of interest within those assemblies through annotations.

Latest research

Microbiology_Metagenomics_STILLS_01Webinar

A route to clinical fungal pathogen sequencing

Join this webinar to learn out about the advantages of targeted ITS and whole-genome sequencing approaches for potential clinical and epidemiological use.
Microbiology_MetagenomicsWebinar

A route to clinical fungal pathogen sequencing

In this webinar, we learn out about the advantages of targeted ITS and whole-genome sequencing approaches for potential clinical and epidemiological use.
ONT_Microbiology_Metagenomics_STILLS_05Publication

Clinical accuracy and operational evaluation of a novel nanopore based 16S diagnostic pathogen detection workflow

Authors: Thomas J. Saville, Magdalena B. Dabrowska, Peijun Zhang, Joanne Bullivant, Amy State, Jack Goodall, Sharon Hsu, Katie Johnson, Christopher Lynch, Rob Townsend, Gayti Morris, Helena Parsons, David Partridge, Thushan de Silva
plasmidWebinar

Strengthening MRSA genomic surveillance in the Pacific

Discover how strengthening local sequencing and bioinformatics capacity can support sustainable pathogen surveillance and informed public health responses across the Pacific.
Publication

Linkage blocks involving multiple replicons organize Borrelia burgdorferi strain variation and influence virulence in Lyme disease

Authors: Rachel A. Laing, Michael J. Foster, M. Amine Hassani, Benjamin Kotzen et al.

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