Metagenomics
Getting started guides
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A guide to microbial sequencing with Oxford Nanopore
A guide to get started with sequencing microbial samples with Oxford Nanopore.
Workflow overviews
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Rapid viral sequencing for genomic surveillance of mpox using metagenomic or targeted approaches
This end-to-end workflow provides guidance for both metagenomic and targeted viral genome sequencing to deliver rapid and actionable results.
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Rapid identification of respiratory pathogens with Oxford Nanopore metagenomics
This end-to-end workflow introduces how to rapidly identify bacterial, fungal, and viral pathogens from respiratory research samples using metagenomic Oxford Nanopore sequencing on a MinION or GridION.
Most viewed
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Oxford Nanopore sequencing provides superior metagenome-assembled genome recovery and strain-level resolution from a complex microbiome
In this application note, we demonstrate the
capabilities of Oxford Nanopore metagenomics by
sequencing and analysing the well-characterised
ZymoBIOMICS Fecal Reference.
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Oxford Nanopore sequencing solutions for microbiology and infectious disease research
Discover the Oxford Nanopore sequencing techniques that can achieve comprehensive microbial genome characterisation.
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EPI2ME: data analysis for all levels of expertise
Discover EPI2ME and how it provides data analysis for all levels of expertise
Featured resources
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Genomics for a changing planet: sequencing the living world
Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.
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Addressing the challenges of metagenomics with Oxford Nanopore sequencing
Explore how reads with unrestricted length are revealing unprecedented insight into microbial communities.
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High-quality bacterial genomes without the complexity
In this case study, researchers show that near-complete bacterial genomes can be assembled using nanopore sequencing alone.
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From full-length 16S to high-resolution metagenomics: how do I choose the right microbial community workflow for my experiment?
In this masterclass, find the right microbial community sequencing workflow to meet your experimental goals. We’ll cover microbial identification from full-length 16S/ITS sequencing through to comprehensive metagenomic assembly. In this masterclass, discover: • How Oxford Nanopore sequencing deliver
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How do I analyse my Oxford Nanopore sequencing data without specialist training?
In this beginner-friendly masterclass, discover how to analyse your Oxford Nanopore sequencing data. Find out how basecalling works, the common file formats you’ll encounter, and how to use MinKNOW to set up and monitor your sequencing run. Then, discover how to analyse your data with EPI2ME — no pr
Protocols
使用 SQK-RPB114.24 进行细菌、真菌和病毒病原体监测的快速宏基因组测序
本实验指南提供了一种快速的宏基因组测序方法，用于鉴定细菌、真菌和病毒性病原体。
此指南具有以下特点：
- 采用二合一流程，可将同一样品分别进行细菌/真菌与病毒/非细胞前处理；
- 提供针对呼吸道样品的宿主去除与核酸提取方法；
- 采用 RT-PCR “鸟枪法”策略；
- 包含酶切法片段化、条形码标记及 PCR 扩增步骤；
- 适用于 R10.4.1 测序芯片。
仅供研究使用
Analysis workflows
wf-amplicon
This Nextflow workflow provides a simple way to analyse Oxford Nanopore reads generated from haploid amplicons.
Latest research
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Unlocking high-resolution, strain-resolved microbiome analyses | ASM Microbe 26
During this video we learn how full length Oxford Nanopore sequencing is unlocking high-resolution microbiome analyses
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Building sustainable pathogen genomic surveillance across Africa
This webinar shares practical lessons from implementing surveillance at scale, to specimen collection and sequencing to bioinformatics and data interpretation. Explore real-world challenges, collaboration models and system-level strategies required to build resilient genomic surveillance.
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Rapid diagnosis of common, undetected, and uncultivable bloodstream infections from positive blood cultures using Oxford Nanopore sequencing: a metagenomic pipeline analysis
Publication: Rapid diagnosis of common, undetected, and uncultivable bloodstream infections from positive blood cultures using Oxford Nanopore sequencing: a metagenomic pipeline analysis
Implementación de la secuenciación de próxima generación en el laboratorio de microbiología
Explore cómo las tecnologías de secuenciación de largos fragmentos de ADN están moldeando las aplicaciones actuales y allanando el camino para futuros avances en metagenómica.
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High-resolution metagenome assembly for modern long reads with myloasm
Publication: High-resolution metagenome assembly for modern long reads with myloasm
