From portable to high-throughput benchtop devices, real-time nanopore sensing is a new generation of technology uncovering new biology across multiple sectors.
21st August 2024
- Added warning to the "Post-pull-down PCR" step to ensure users DO NOT allow the amplicon-bead conjugate to pellet before incubating.
- NEB reagent recommendations for purchase and use updated (Salt T4 ligase in place of Quick Ligation Module).
- Updated the Adapter ligation step with new recommended method.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
30 July 2024
- Updated wording in protocol introduction and in equipment and consumables: "This protocol is compatible and fully supported with 10X Genomics Next GEM Single Cell 3' Kit (V3.1) and the Visium Spatial Gene Expression Kit (V1). Other versions of the kits are not supported."
30 July 2024
- Updated wording in protocol introduction and in equipment and consumables: "This protocol is compatible and fully supported with 10X Genomics Next GEM Single Cell 3' Kit (V3.1) and the Visium Spatial Gene Expression Kit (V1). Other versions of the kits are not supported."