Ligation sequencing V14 - PCR barcoding (SQK-LSK114 with EXP-PBC001 or EXP-PBC096)

DateVersion numberLanguageDeviceChanges
Aug 21 2024PBC_9182_v114_revK_07Mar2023EnglishGridION
21st August 2024 - Updated protocol introduction to new format. - Updated sample input recommendations to 100 ng for gDNA or amplicons. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
Aug 21 2024PBC_9182_v114_revK_07Mar2023EnglishMinION
21st August 2024 - Updated protocol introduction to new format. - Updated sample input recommendations to 100 ng for gDNA or amplicons. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
Aug 21 2024PBC_9182_v114_revK_07Mar2023EnglishPromethION
21st August 2024 - Updated protocol introduction to new format. - Updated sample input recommendations to 100 ng for gDNA or amplicons. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
August 21st, 2024PBC_9182_v114_revJ_07Mar2023EnglishPromethion Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024PBC_9182_v114_revJ_07Mar2023EnglishGridion Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024PBC_9182_v114_revJ_07Mar2023EnglishMinion Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 31st, 2024PBC_9182_v114_revI_07Mar2023EnglishPromethion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 31st, 2024PBC_9182_v114_revI_07Mar2023EnglishGridion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 31st, 2024PBC_9182_v114_revI_07Mar2023EnglishMinion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
September 19th, 2023PBC_9182_v114_revG_07Mar2023EnglishPromethion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated - Added amplicon tailed primer sequences to the introduction
September 19th, 2023PBC_9182_v114_revG_07Mar2023EnglishGridion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated - Added amplicon tailed primer sequences to the introduction
September 19th, 2023PBC_9182_v114_revG_07Mar2023EnglishMinion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated - Added amplicon tailed primer sequences to the introduction
May 3rd, 2023PBC_9182_v114_revF_07Mar2023EnglishPromethion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
May 3rd, 2023PBC_9182_v114_revF_07Mar2023EnglishGridion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
May 3rd, 2023PBC_9182_v114_revF_07Mar2023EnglishMinion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
April 28th, 2023PBC_9182_v114_revE_07Mar2023EnglishPromethion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023PBC_9182_v114_revE_07Mar2023EnglishGridion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023PBC_9182_v114_revE_07Mar2023EnglishMinion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
March 24th, 2023PBC_9182_v114_revD_07Mar2023EnglishPromethion Download March 2023: - Updated how much DNA is expected and taken forwards from the first end-prep step
March 24th, 2023PBC_9182_v114_revD_07Mar2023EnglishGridion Download March 2023: - Updated how much DNA is expected and taken forwards from the first end-prep step
March 24th, 2023PBC_9182_v114_revD_07Mar2023EnglishMinion Download March 2023: - Updated how much DNA is expected and taken forwards from the first end-prep step
March 17th, 2023PBC_9182_v114_revC_07Mar2023EnglishPromethion Download March 2023: - Added instructions for preparing ONT reagents
March 17th, 2023PBC_9182_v114_revC_07Mar2023EnglishGridion Download March 2023: - Added instructions for preparing ONT reagents
March 17th, 2023PBC_9182_v114_revC_07Mar2023EnglishMinion Download March 2023: - Added instructions for preparing ONT reagents
March 16th, 2023PBC_9182_v114_revB_07Mar2023EnglishPromethion Download March 2023: - Updated input recommendations for gDNA in the "Equipment and consumables" section, as well as in the "End-prep" step.
March 16th, 2023PBC_9182_v114_revB_07Mar2023EnglishGridion Download March 2023: - Updated input recommendations for gDNA in the "Equipment and consumables" section, as well as in the "End-prep" step.
March 16th, 2023PBC_9182_v114_revB_07Mar2023EnglishMinion Download March 2023: - Updated input recommendations for gDNA in the "Equipment and consumables" section, as well as in the "End-prep" step.
March 8th, 2023PBC_9182_v114_revA_07Mar2023EnglishPromethion Download
March 8th, 2023PBC_9182_v114_revA_07Mar2023EnglishGridion Download
March 8th, 2023PBC_9182_v114_revA_07Mar2023EnglishMinion Download