Solución de secuenciación de aislados microbianos exclusivo de Nanopore (NO-MISS) a partir de cultivos celulares utilizando SQK-RBK114 (.24 o .96) (ISO_9205_v114_revH_12Dec2025)
12th December 2025
-- In 'Overview of the protocol', in the Table under 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min.
- In 'Compatibility of this protocol', MinION Mk1B was removed, along with its link for IT requirements as this device document has moved to legacy.
- In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was corrected.
- In section 4, 'Library preparation' , 'Check your flow cell' and section 5, 'Priming and loading the flow cell', 'Complete a flow cell check', the link for the flow cell check in the MinKNOW protocol was replaced with a link for the flow cell check document. And in section 4, a note was added to use the barcode wells only once.
- In section 5, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- In 'Data acquisition and basecalling', screenshots for MinKNOW were removed.
12/12/2025
ISO_9205_v114_revH_12Dec2025
English
MinION
12th December 2025
-- In 'Overview of the protocol', in the Table under 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min.
- In 'Compatibility of this protocol', MinION Mk1B was removed, along with its link for IT requirements as this device document has moved to legacy.
- In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was corrected.
- In section 4, 'Library preparation' , 'Check your flow cell' and section 5, 'Priming and loading the flow cell', 'Complete a flow cell check', the link for the flow cell check in the MinKNOW protocol was replaced with a link for the flow cell check document. And in section 4, a note was added to use the barcode wells only once.
- In section 5, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- In 'Data acquisition and basecalling', screenshots for MinKNOW were removed.
12/12/2025
ISO_9205_v114_revH_12Dec2025
Español
MinION
12th December 2025
-- In 'Overview of the protocol', in the Table under 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min.
- In 'Compatibility of this protocol', MinION Mk1B was removed, along with its link for IT requirements as this device document has moved to legacy.
- In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was corrected.
- In section 4, 'Library preparation' , 'Check your flow cell' and section 5, 'Priming and loading the flow cell', 'Complete a flow cell check', the link for the flow cell check in the MinKNOW protocol was replaced with a link for the flow cell check document. And in section 4, a note was added to use the barcode wells only once.
- In section 5, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- In 'Data acquisition and basecalling', screenshots for MinKNOW were removed.
6/23/2025
ISO_9205_v114_revG_09May2025
English
GridION
09 May 2025
- Protocol title update
6/23/2025
ISO_9205_v114_revG_09May2025
English
MinION
09 May 2025
- Protocol title update
12/13/2024
ISO_9205_v114_revF_13Dec2024
English
GridION
13 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024
ISO_9205_v114_revF_13Dec2024
English
MinION
13 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024
ISO_9205_v114_revE_20Nov2024
English
GridION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
11/15/2024
ISO_9205_v114_revE_20Nov2024
English
MinION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
10/14/2024
ISO_9205_v114_revD_14Oct2024
English
GridION
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
10/14/2024
ISO_9205_v114_revD_14Oct2024
English
MinION
14th October 2024
- Updated content nomenclature to reflect the updated product name for Monarch Genomic DNA Purification Kit #T3010. This has now changed to Monarch® Spin gDNA Extraction Kit #T3010.
- Components within the Monarch® Spin gDNA Extraction Kit #T3010 have also had updated names and product codes:
| Old name and code | New name/subcomponent part number |
| --- | --- |
| Monarch® gDNA Purification Columns T3017-1 | Monarch® Spin Columns S2C and Tubes T3017-1 |
| Monarch® Collection Tubes II T2018-1 | Monarch® Spin Collection Tubes T2118-1 |
| Proteinase K, Molecular Biology Grade P8107AAVIAL | Proteinase K, Molecular Biology Grade P8200AAVIAL |
9/30/2024
ISO_9205_v114_revC_30Sept2024
English
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
ISO_9205_v114_revC_30Sept2024
English
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
5/14/2024
ISO_9205_v114_revB_20Mar2024
English
GridION
27th March 2024
- Updated wording for MinKNOW updates to specify v24.02 or newer
5/14/2024
ISO_9205_v114_revB_20Mar2024
English
MinION
27th March 2024
- Updated wording for MinKNOW updates to specify v24.02 or newer
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