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Plan
This section will help you plan your nanopore sequencing experiment end-to-end.
Methodology best practice
Adaptive sampling
Last updated 10/16/2024
Prepare
Preparation for sequencing involves extracting the DNA/RNA from your sample, optional fragmentation/size selection steps, and preparing your library using one of Oxford Nanopore's kits.
Extraction protocols
SPRI size selection protocol for >1.5-2 kb DNA fragments
Last updated 11/11/2024
Sequence
This section provides technical specifications for our sequencing devices, and instructions for how to install and run them.
Device installation & configuration
MinION Mk1D user manual
Last updated 11/6/2024
Sequencing software
MinKNOW
Last updated 11/22/2024
Analyse
Nanopore data analysis involves acquisition of raw signal data from the sequencing device, basecalling, and post-basecalling analysis.
Basecalling
Kit 14 sequencing and duplex basecalling
Last updated 10/8/2024
EPI2ME workflows
Workflow: Transcriptomes [wf-transcriptomes]
Last updated 3/28/2024
Application workflows
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Posts
Interact with thousands of other nanopore sequencers from around the globe.
Private Channels
Discuss specific topics and technology applications.
Product and technology updates
Stay up to date with the latest Oxford Nanopore products and technology.
Software
Download latest versions of sequencing and analysis software.