Fusion transcripts
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Full-length sequencing of fusion transcripts
The accurate characterisation of fusion transcripts is of high importance for clinical research into diseases, including some forms of cancer. Confident identification of fusion transcripts requires sequencing reads to span the fusion junction and include sufficient sequence on either side for accurate identification.
This capacity is often limited when using legacy short-read sequencing approaches. However, with Oxford Nanopore reads of unrestricted length, fusion transcripts can be sequenced end to end in single reads, enabling comprehensive characterisation of fusions and their precise splice junctions.
Featured content
Characterising somatic structural variation in colorectal cancer with long nanopore reads
In this case study, discover how nanopore sequencing of colorectal cancer research enabled the characterisation of fusions that could have evaded detection with other techniques
The value of full-length transcripts without bias
This white paper introduces the facility of RNA and cDNA nanopore sequencing to deliver full-length transcript information, isoform characterisation and quantification, and RNA virus identification.
Recommended device for fusion transcript sequencing
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MinION
Delivering real-time sequencing anywhere, the portable MinION enables on-demand sequencing of full-length fusion transcripts. Targeted fusion or whole-transcriptome sequencing approaches can be utilised, depending on the goals of an experiment.
