Gene expression
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Full-length transcripts in single reads
Analysis of gene expression is important in many applications, from clinical research to developmental biology. However, the use of legacy short-read technologies can cause multi-mapping when aligning data and limit quantification accuracy; this can be further limited by PCR bias.
In contrast, Oxford Nanopore reads of unrestricted length allow transcripts to be sequenced end to end, enabling accurate quantification and complete characterisation of isoforms in a single dataset. Furthermore, direct RNA sequencing enables the simultaneous detection of epigenetic modifications and eliminates PCR bias.
Featured content
The value of full-length transcripts without bias
This white paper focuses on the facility of nanopore RNA and cDNA sequencing to tackle challenges in the areas of full-length transcript identification, isoform characterisation and quantification, and viral detection.
Direct RNA sequencing workflow
This workflow shares how direct RNA nanopore sequencing delivers full-length transcript sequencing, enabling quantitation of gene and isoform expression without bias and detection of RNA modifications.
High-definition transcriptomics
Recommended device for gene expression studies
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PromethION 2 Integrated and 2 Solo
Gain flexible, high-output nanopore sequencing for every lab with the PromethION 2 devices. Generate high depth of coverage of whole transcriptomes on up to two individually addressable PromethION Flow Cells, enabling high-resolution gene expression analysis.
The PromethION 2 Integrated is a self-contained, benchtop device with powerful onboard compute, while the compact PromethION 2 Solo utilises resource within a GridION or other existing compute.
