Ligation sequencing influenza whole genome V14 (SQK-NBD114.24 or SQK-NBD114.96) (INF_9189_v114_revK_13Dec2024)

DateVersionLanguageDeviceChanges
1/28/2025INF_9189_v114_revK_13Dec2024EnglishGridION
28th January 2025 Small typo correction
1/28/2025INF_9189_v114_revK_13Dec2024EnglishMinION
28th January 2025 Small typo correction
12/13/2024INF_9189_v114_revJ_13Dec2024EnglishGridION
13 December 2024 - Addition of link to MinION Mk1D IT requirements in "Computer requirements and software" and updated link to EPI2ME. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/13/2024INF_9189_v114_revJ_13Dec2024EnglishMinION
13 December 2024 - Addition of link to MinION Mk1D IT requirements in "Computer requirements and software" and updated link to EPI2ME. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024INF_9189_v114_revI_20Nov2024EnglishGridION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/15/2024INF_9189_v114_revI_20Nov2024EnglishMinION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
10/24/2024INF_9189_v114_revH_24Oct2024EnglishGridION
24th October 2024 - Fixed formatting of table in step 4 in "Native barcode ligation".
10/24/2024INF_9189_v114_revH_24Oct2024EnglishMinION
24th October 2024 - Fixed formatting of table in step 4 in "Native barcode ligation".
9/30/2024INF_9189_v114_revG_30Sept2024EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024INF_9189_v114_revG_30Sept2024EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/2024INF_9189_v114_revG_30Sept2024EnglishGridION
30th September 2024 - Removed protocol from early access phase.
9/30/2024INF_9189_v114_revG_30Sept2024EnglishMinION
30th September 2024 - Removed protocol from early access phase.
9/30/2024INF_9189_v114_revG_14Jun2023EnglishGridION
30th September 2024 - Removed protocol from early access phase.
9/30/2024INF_9189_v114_revG_14Jun2023EnglishMinION
30th September 2024 - Removed protocol from early access phase.
3/7/2024INF_9189_v114_revF_14Jun2023EnglishGridION
07th March 2024 - Updated library loading recommendations to 35-50 fmols.
3/7/2024INF_9189_v114_revF_14Jun2023EnglishMinION
07th March 2024 - Updated library loading recommendations to 35-50 fmols.
January 22nd, 2024INF_9189_v114_revD_14Jun2023EnglishGridion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
January 22nd, 2024INF_9189_v114_revD_14Jun2023EnglishMinion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 2nd, 2023INF_9189_v114_revC_14Jun2023EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
October 2nd, 2023INF_9189_v114_revC_14Jun2023EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
September 19th, 2023INF_9189_v114_revB_14Jun2023EnglishGridion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 19th, 2023INF_9189_v114_revB_14Jun2023EnglishMinion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 14th, 2023INF_9189_v114_revA_14Jun2023EnglishGridion Download -
September 14th, 2023INF_9189_v114_revA_14Jun2023EnglishMinion Download -