10th September 2025
In 'Overview of the protocol', in the Table under 'Library preparation', the timing for priming and loading a flow cell was increased from 5 min to 10 min and timings in the workflow image were updated.
- In 'Equipment and consumables', the image of the LSK114 kit in single-use tube format was removed. And instructions for the single-use tube format were removed from section 7, step 2.
- In section 5, 'DNA repair and end-prep', 'Check your flow cell' and section 7, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 5, 'DNA repair and end-prep', step 15, instructions were provided on how to resuspend the AMPure XP bead pellet with nuclease-free water.
- In section 7, 'Priming and loading the flow cell', the instruction for removing the flow cell out of the fridge was moved to the top of this section.
- In 'Troubleshooting', section 12, the videos links for priming and loading a MinION Flow Cell were replaced with links for the PromethION Flow Cell.
1/22/2025
GDH_9173_v114_revQ_22Jan2025
English
PromethION
22 January 2025
- Document title change
- gDNA Extraction: method updated
8/23/2024
GDH_9173_v114_revP_10Nov2022
English
PromethION
23st August 2024
- Reverted the Sample preparation section to Human cell line DNA extraction using the QIAGEN Puregene Cell Kit & Shearing DNA for 10 kb input using the Covaris g-TUBE™
- Sample input recommendation into library preparation reverted to 1 ug.
21st August 2024
- Updated workflow overview image.
- Updated sample preparation instructions.
- Updated sample input recommendation into library preparation.
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Data acquisition and basecalling instructions updated.
- Downstream analysis section updated.
23st August 2024
- Reverted the Sample preparation section to Human cell line DNA extraction using the QIAGEN Puregene Cell Kit & Shearing DNA for 10 kb input using the Covaris g-TUBE™
- Sample input recommendation into library preparation reverted to 1 ug.
21st August 2024
- Updated workflow overview image.
- Updated sample preparation instructions.
- Updated sample input recommendation into library preparation.
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Data acquisition and basecalling instructions updated.
- Downstream analysis section updated.
21st August 2024
- Updated workflow overview image.
- Updated sample preparation instructions.
- Updated sample input recommendation into library preparation.
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Data acquisition and basecalling instructions updated.
- Downstream analysis section updated.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
30 April 2024
- Updated 'Downstream analysis' instructions.
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
10th July 2023
- Updated "Data acquisition and basecalling" to use the most recent MinKNOW GUI version in screenshots
- Updated "Downstream analysis" with the most recent recommendations from EPI2ME labs to ensure successful sequencing and data analysis
- Updated recommendation in the "Downstream analysis" to use modkit not modbam2bed has been depricated.
10th July 2023
- Updated "Data acquisition and basecalling" to use the most recent MinKNOW GUI version in screenshots
- Updated "Downstream analysis" with the most recent recommendations from EPI2ME labs to ensure successful sequencing and data analysis
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