Direct-from-colony microbial sequencing: rapid Salmonella serotyping (SQK-RPB114.24) (DCM_9210_V114_revC_11Dec2024)
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- Direct-from-colony microbial sequencing: rapid Salmonella serotyping (SQK-RPB114.24)
Date | Version | Language | Device | Changes | |
---|---|---|---|---|---|
12/11/2024 | DCM_9210_V114_revC_11Dec2024 | English | GridION | 11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature". | |
12/11/2024 | DCM_9210_V114_revC_11Dec2024 | English | MinION | 11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature". | |
9/30/2024 | DCM_9210_V114_revB_30Sep2024 | English | GridION | 30th September 2024 - Included biosafety warning for pathogenic organisms and recommendation to use appropriate laboratory equipment to protocol introduction. - Incuded biosafety warnings in the preparation method. - Included recommendations to protocol introduction for sample storage following PCR amplification. - Updated PCR step to include EDTA quench reaction following amplification. | |
9/30/2024 | DCM_9210_V114_revB_30Sep2024 | English | MinION | 30th September 2024 - Included biosafety warning for pathogenic organisms and recommendation to use appropriate laboratory equipment to protocol introduction. - Incuded biosafety warnings in the preparation method. - Included recommendations to protocol introduction for sample storage following PCR amplification. - Updated PCR step to include EDTA quench reaction following amplification. | |
9/4/2024 | DCM_9210_V114_revA_04Sep2024 | English | GridION | 04th September 2024 Protocol release | |
9/4/2024 | DCM_9210_V114_revA_04Sep2024 | English | MinION | 04th September 2024 Protocol release |