Direct-from-colony microbial sequencing: rapid Salmonella serotyping (SQK-RPB114.24) (DCM_9210_V114_revC_11Dec2024)


DateVersionLanguageDeviceChanges
12/11/2024DCM_9210_V114_revC_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DCM_9210_V114_revC_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
9/30/2024DCM_9210_V114_revB_30Sep2024EnglishGridION
30th September 2024 - Included biosafety warning for pathogenic organisms and recommendation to use appropriate laboratory equipment to protocol introduction. - Incuded biosafety warnings in the preparation method. - Included recommendations to protocol introduction for sample storage following PCR amplification. - Updated PCR step to include EDTA quench reaction following amplification.
9/30/2024DCM_9210_V114_revB_30Sep2024EnglishMinION
30th September 2024 - Included biosafety warning for pathogenic organisms and recommendation to use appropriate laboratory equipment to protocol introduction. - Incuded biosafety warnings in the preparation method. - Included recommendations to protocol introduction for sample storage following PCR amplification. - Updated PCR step to include EDTA quench reaction following amplification.
9/4/2024DCM_9210_V114_revA_04Sep2024EnglishGridION
04th September 2024 Protocol release
9/4/2024DCM_9210_V114_revA_04Sep2024EnglishMinION
04th September 2024 Protocol release