Resolving structural variants with long nanopore sequencing reads

Overview

Structural variants (SVs) have known causative effects in an extensive range of both normal and aberrant phenotypes. Using PCR-free nanopore sequencing, long nanopore reads can span entire SVs in a single read, including within repetitive or GC-rich regions for accurate resolution of even highly complex variants in any genomic context. This end-to-end workflow provides a simple method for an effective whole-genome SV survey from a human blood research sample.

In this workflow, you will:

• Find out how direct nanopore sequencing enhances the resolution of complex SVs
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices