29th October 2025
- In section 1, 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 2, 'Equipment and consumables', image of the kit in the single-use tube format was removed.
- The section 'Computer requirements and software' was removed as the information is available in section 1, 'Compatibility of this protocol'.
- In section 3, 'Library preparation' a note was added to check your flow cell.
- In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. Instructions for the use of the kit in tube format were removed from step 2. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
10/29/2025
RSL_9176_v114_revE_29Oct2025
English
MinION
29th October 2025
- In section 1, 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 2, 'Equipment and consumables', image of the kit in the single-use tube format was removed.
- The section 'Computer requirements and software' was removed as the information is available in section 1, 'Compatibility of this protocol'.
- In section 3, 'Library preparation' a note was added to check your flow cell.
- In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. Instructions for the use of the kit in tube format were removed from step 2. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
12/11/2024
RSL_9176_v114_revD_11Dec2024
English
GridION
11 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024
RSL_9176_v114_revD_11Dec2024
English
MinION
11 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/8/2024
RSL_9176_v114_revC_16Nov2022
English
GridION
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
19th September 2023
- Updated flow cell loading instructions to include light shield installation
8/8/2024
RSL_9176_v114_revC_16Nov2022
English
MinION
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
19th September 2023
- Updated flow cell loading instructions to include light shield installation
