From portable to high-throughput benchtop devices, real-time nanopore sensing is a new generation of technology uncovering new biology across multiple sectors.
21st August 2024
- Updated sample input recommendations with table for different DNA size.
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
Aug 21 2024
CDE_9162_v114_revT_29Jun2022
English
GridION
21st August 2024
- Updated sample input recommendations with table for different DNA size.
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
Aug 21 2024
CDE_9162_v114_revT_29Jun2022
English
MinION
21st August 2024
- Updated sample input recommendations with table for different DNA size.
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
Aug 21 2024
CDE_9162_v114_revT_29Jun2022
English
PromethION
21st August 2024
- Updated sample input recommendations with table for different DNA size.
- NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module.
- Updated the Repair and End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Updated elution step in "Adapter ligation and clean-up" section to specify 10 minute incubation for PromethION branch.
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Updated elution step in "Adapter ligation and clean-up" section to specify 10 minute incubation for PromethION branch.
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Updated elution step in "Adapter ligation and clean-up" section to specify 10 minute incubation for PromethION branch.
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
18th April 2024
- Updated elution step in "Adapter ligation and clean-up" section to specify 10 minute incubation for PromethION branch.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated