11th November 2025
- In section 1, in the Table under 'Library preparation', the timing for the priming and loading a flow cell was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B was removed, along with its link for IT requirements as this device document has moved to legacy.
- In section 3, 'Library preparation', a note was added to check your flow cell and to use the barcode wells only once.
- In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- Links in section 6, 'Downstream analysis' were updated.
11/11/2025
DCM_9210_V114_revF_11Nov2025
English
MinION
11th November 2025
- In section 1, in the Table under 'Library preparation', the timing for the priming and loading a flow cell was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B was removed, along with its link for IT requirements as this device document has moved to legacy.
- In section 3, 'Library preparation', a note was added to check your flow cell and to use the barcode wells only once.
- In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- Links in section 6, 'Downstream analysis' were updated.
5/9/2025
DCM_9210_V114_revE_09May2025
English
GridION
09th May 2025
- Added information for SQK-RPB114.24 kit format update from vials to plate.
- Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
- Protocol title update
5/9/2025
DCM_9210_V114_revE_09May2025
English
MinION
09th May 2025
- Added information for SQK-RPB114.24 kit format update from vials to plate.
- Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
- Protocol title update
12/11/2024
DCM_9210_V114_revC_11Dec2024
English
GridION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024
DCM_9210_V114_revC_11Dec2024
English
MinION
11th December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
9/30/2024
DCM_9210_V114_revB_30Sep2024
English
GridION
30th September 2024
- Included biosafety warning for pathogenic organisms and recommendation to use appropriate laboratory equipment to protocol introduction.
- Incuded biosafety warnings in the preparation method.
- Included recommendations to protocol introduction for sample storage following PCR amplification.
- Updated PCR step to include EDTA quench reaction following amplification.
9/30/2024
DCM_9210_V114_revB_30Sep2024
English
MinION
30th September 2024
- Included biosafety warning for pathogenic organisms and recommendation to use appropriate laboratory equipment to protocol introduction.
- Incuded biosafety warnings in the preparation method.
- Included recommendations to protocol introduction for sample storage following PCR amplification.
- Updated PCR step to include EDTA quench reaction following amplification.
