A guide to sequencing large genomes with Oxford Nanopore
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Overview
Plant and animal genomes are incredibly diverse with up to a 10,000-fold difference in lengths across many species, as well as variation in ploidy increasing their genome complexity. Legacy short-read sequencing technologies are limited in their ability to sequence complete genomes of many known plant and animal species due to their large genome size and complexity.
In this getting started guide, find out how nanopore reads of any length overcome these limitations to sequence large and complex genomes in fewer fragments with greater overlap for easier genome assembly.
In this getting started guide, you will:
- Find out how nanopore reads of any length enhance whole-genome sequencing of large genomes
- Discover the benefits of nanopore sequencing
- Identify the appropriate nanopore sequencing kit for your experiment
- For any questions, see the FAQs for this technique
- Read real-world case studies of how researchers are utilising nanopore sequencing to simplify genome assembly and sequence more complete plant and animal genomes