1st November 2024
- Updated "Overview of the protocol": Removal of reference to Herro within the "Sequencing and analysis" section in reference to "Assembly Polishing Kit experiment". Now references use of Dorado for read correction.
- Included instructions for obtaining and installing the SQK-APK114 sequencing script on your device in the "Data acquisition and basecalling: Assembly Polishing Kit" section of the protocol.
- Updated formatting for the "Data acquisition and basecalling" pages of the protocol to more easily separate out the parameters of each section.
Nov 1 2024
T2T_9211_v114_revD_04Sep2024
English
PromethION
1st November 2024
- Updated "Overview of the protocol": Removal of reference to Herro within the "Sequencing and analysis" section in reference to "Assembly Polishing Kit experiment". Now references use of Dorado for read correction.
- Included instructions for obtaining and installing the SQK-APK114 sequencing script on your device in the "Data acquisition and basecalling: Assembly Polishing Kit" section of the protocol.
- Updated formatting for the "Data acquisition and basecalling" pages of the protocol to more easily separate out the parameters of each section.
Sep 24 2024
T2T_9211_v114_revC_04Sep2024
English
PromethION
24th September 2024
- Medaka model pinned to version 1.12.1 to avoid discrepancy issues in downstream analysis.
Sep 10 2024
T2T_9211_v114_revB_04Sep2024
English
PromethION
10th September 2024
- Updated setting recommendations in downstream analysis.