Ligation sequencing gDNA - Native Barcoding Kit 24 V14 (SQK-NBD114.24) (NBE_9169_v114_revU_30Jan2025)

DateVersionLanguageDeviceChanges
1/30/2025NBE_9169_v114_revU_30Jan2025EnglishFlongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025EnglishGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025EnglishMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025EnglishPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025中文Flongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025中文GridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025中文MinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025中文PromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025EnglishFlongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025EnglishGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025EnglishMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025EnglishPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025中文Flongle
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025中文GridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025中文MinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025NBE_9169_v114_revU_30Jan2025中文PromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/27/2024NBE_9169_v114_revT_27Dec2024EnglishFlongle
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024NBE_9169_v114_revT_27Dec2024EnglishGridION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024NBE_9169_v114_revT_27Dec2024EnglishMinION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024NBE_9169_v114_revT_27Dec2024EnglishPromethION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024NBE_9169_v114_revT_27Dec2024中文Flongle
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024NBE_9169_v114_revT_27Dec2024中文GridION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024NBE_9169_v114_revT_27Dec2024中文MinION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/27/2024NBE_9169_v114_revT_27Dec2024中文PromethION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revT_19Dec2024EnglishFlongle
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revT_19Dec2024EnglishGridION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revT_19Dec2024EnglishMinION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revT_19Dec2024EnglishPromethION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revT_19Dec2024中文Flongle
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revT_19Dec2024中文GridION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revT_19Dec2024中文MinION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revT_19Dec2024中文PromethION
19 December 2024 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024EnglishFlongle
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024EnglishGridION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024EnglishMinION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024EnglishPromethION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024中文Flongle
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024中文GridION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024中文MinION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024中文PromethION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024EnglishFlongle
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024EnglishGridION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024EnglishMinION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024EnglishPromethION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024中文Flongle
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024中文GridION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024中文MinION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
12/19/2024NBE_9169_v114_revS_27Nov2024中文PromethION
December 2024/January 2025 - Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing". - Updated link for EPI2ME in "Computer requirements and software", "EPI2ME (optional)". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the SpotON flow cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
11/14/2024NBE_9169_v114_revS_20Nov2024EnglishFlongle
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/14/2024NBE_9169_v114_revS_20Nov2024EnglishGridION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/14/2024NBE_9169_v114_revS_20Nov2024EnglishMinION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/14/2024NBE_9169_v114_revS_20Nov2024EnglishPromethION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/14/2024NBE_9169_v114_revS_20Nov2024中文Flongle
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/14/2024NBE_9169_v114_revS_20Nov2024中文GridION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/14/2024NBE_9169_v114_revS_20Nov2024中文MinION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
11/14/2024NBE_9169_v114_revS_20Nov2024中文PromethION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
8/21/2024NBE_9169_v114_revR_15Sep2022EnglishFlongle
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
8/21/2024NBE_9169_v114_revR_15Sep2022EnglishGridION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
8/21/2024NBE_9169_v114_revR_15Sep2022EnglishMinION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
8/21/2024NBE_9169_v114_revR_15Sep2022EnglishPromethION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
8/21/2024NBE_9169_v114_revR_15Sep2022中文Flongle
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
8/21/2024NBE_9169_v114_revR_15Sep2022中文GridION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
8/21/2024NBE_9169_v114_revR_15Sep2022中文MinION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
8/21/2024NBE_9169_v114_revR_15Sep2022中文PromethION
21st August 2024 - Removed Early access loading now that kit is in "released" phase. - Removed reference to duplex sequencing. - Updated flow cell loading recommendations based off input fragment size.
August 21st, 2024NBE_9169_v114_revQ_15Sep2022EnglishFlongle Download 3rd November 2023 - Corrected duplication of Sequencing and Data analysis section
August 21st, 2024NBE_9169_v114_revQ_15Sep2022EnglishPromethion Download 3rd November 2023 - Corrected duplication of Sequencing and Data analysis section
August 21st, 2024NBE_9169_v114_revQ_15Sep2022EnglishGridion Download 3rd November 2023 - Corrected duplication of Sequencing and Data analysis section
August 21st, 2024NBE_9169_v114_revQ_15Sep2022EnglishMinion Download 3rd November 2023 - Corrected duplication of Sequencing and Data analysis section
November 3rd, 2023NBE_9169_v114_revP_15Sep2022EnglishFlongle Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
November 3rd, 2023NBE_9169_v114_revP_15Sep2022EnglishPromethion Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
November 3rd, 2023NBE_9169_v114_revP_15Sep2022EnglishGridion Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
November 3rd, 2023NBE_9169_v114_revP_15Sep2022EnglishMinion Download 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
October 4th, 2023NBE_9169_v114_revO_15Sep2022EnglishFlongle Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 4th, 2023NBE_9169_v114_revO_15Sep2022EnglishPromethion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 4th, 2023NBE_9169_v114_revO_15Sep2022EnglishGridion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 4th, 2023NBE_9169_v114_revO_15Sep2022EnglishMinion Download 2nd October 2023 - Updated "Adapter ligation and clean-up" to remove additional reference of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058)
October 2nd, 2023NBE_9169_v114_revN_15Sep2022EnglishFlongle Download 26th September 2023 - Added duplex information to the Sequencing and data analysis section
October 2nd, 2023NBE_9169_v114_revN_15Sep2022EnglishPromethion Download 26th September 2023 - Added duplex information to the Sequencing and data analysis section
October 2nd, 2023NBE_9169_v114_revN_15Sep2022EnglishGridion Download 26th September 2023 - Added duplex information to the Sequencing and data analysis section
October 2nd, 2023NBE_9169_v114_revN_15Sep2022EnglishMinion Download 26th September 2023 - Added duplex information to the Sequencing and data analysis section
September 26th, 2023NBE_9169_v114_revM_15Sep2022EnglishFlongle Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Format change in "Native barcode ligation" step to fix downloaded checklist.
September 26th, 2023NBE_9169_v114_revM_15Sep2022EnglishPromethion Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Format change in "Native barcode ligation" step to fix downloaded checklist.
September 26th, 2023NBE_9169_v114_revM_15Sep2022EnglishGridion Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Format change in "Native barcode ligation" step to fix downloaded checklist.
September 26th, 2023NBE_9169_v114_revM_15Sep2022EnglishMinion Download 21st September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Format change in "Native barcode ligation" step to fix downloaded checklist.
September 19th, 2023NBE_9169_v114_revK_15Sep2022EnglishFlongle Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 19th, 2023NBE_9169_v114_revK_15Sep2022EnglishPromethion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 19th, 2023NBE_9169_v114_revK_15Sep2022EnglishGridion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 19th, 2023NBE_9169_v114_revK_15Sep2022EnglishMinion Download 14th August 2023 - Updated the equipment and consumables with both formats of kits which are being shipped. - Updated the native barcoding ligation step to include instructions for both EDTA concentrations. - Updated flow cell flushing instructions to include instructions for the bottle format kit.
September 14th, 2023NBE_9169_v114_revJ_15Sep2022EnglishFlongle Download 1st August 2023 - Updated recommended magnetic rack
September 14th, 2023NBE_9169_v114_revJ_15Sep2022EnglishPromethion Download 1st August 2023 - Updated recommended magnetic rack
September 14th, 2023NBE_9169_v114_revJ_15Sep2022EnglishGridion Download 1st August 2023 - Updated recommended magnetic rack
September 14th, 2023NBE_9169_v114_revJ_15Sep2022EnglishMinion Download 1st August 2023 - Updated recommended magnetic rack
August 1st, 2023NBE_9169_v114_revI_15Sep2022EnglishFlongle Download 30th June 2023 - Added Flongle branch to the protocol.
August 1st, 2023NBE_9169_v114_revI_15Sep2022EnglishPromethion Download 30th June 2023 - Added Flongle branch to the protocol.
August 1st, 2023NBE_9169_v114_revI_15Sep2022EnglishGridion Download 30th June 2023 - Added Flongle branch to the protocol.
August 1st, 2023NBE_9169_v114_revI_15Sep2022EnglishMinion Download 30th June 2023 - Added Flongle branch to the protocol.
April 24th, 2023NBE_9169_v114_revH_15Sep2022EnglishPromethion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 24th, 2023NBE_9169_v114_revH_15Sep2022EnglishGridion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 24th, 2023NBE_9169_v114_revH_15Sep2022EnglishMinion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 5th, 2023NBE_9169_v114_revG_15Sep2022EnglishPromethion Download April 2023 - Updated the DNA repair end-prep step mastermix tip.
April 5th, 2023NBE_9169_v114_revG_15Sep2022EnglishGridion Download April 2023 - Updated the DNA repair end-prep step mastermix tip.
April 5th, 2023NBE_9169_v114_revG_15Sep2022EnglishMinion Download April 2023 - Updated the DNA repair end-prep step mastermix tip.
March 24th, 2023NBE_9169_v114_revF_15Sep2022EnglishPromethion Download March 2023 - Updated DNA repair and end-prep step to show the tip for making up an end-prep mastermix before combining the reagents. - Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 24th, 2023NBE_9169_v114_revF_15Sep2022EnglishGridion Download March 2023 - Updated DNA repair and end-prep step to show the tip for making up an end-prep mastermix before combining the reagents. - Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 24th, 2023NBE_9169_v114_revF_15Sep2022EnglishMinion Download March 2023 - Updated DNA repair and end-prep step to show the tip for making up an end-prep mastermix before combining the reagents. - Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
January 6th, 2023NBE_9169_v114_revE_15Sep2022EnglishPromethion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023NBE_9169_v114_revE_15Sep2022EnglishGridion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
January 6th, 2023NBE_9169_v114_revE_15Sep2022EnglishMinion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
October 20th, 2022NBE_9169_v114_revD_15Sep2022EnglishPromethion Download 20th October 2022 - EXP-NBA114 information updated with availability
October 20th, 2022NBE_9169_v114_revD_15Sep2022EnglishGridion Download 20th October 2022 - EXP-NBA114 information updated with availability
October 20th, 2022NBE_9169_v114_revD_15Sep2022EnglishMinion Download 20th October 2022 - EXP-NBA114 information updated with availability
October 18th, 2022NBE_9169_v114_revC_15Sep2022EnglishPromethion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 18th, 2022NBE_9169_v114_revC_15Sep2022EnglishGridion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 18th, 2022NBE_9169_v114_revC_15Sep2022EnglishMinion Download 18th October 2022 - Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
October 4th, 2022NBE_9169_v114_revB_15Sep2022EnglishPromethion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022NBE_9169_v114_revB_15Sep2022EnglishGridion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022NBE_9169_v114_revB_15Sep2022EnglishMinion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
September 15th, 2022NBE_9169_v114_revA_15Sep2022EnglishPromethion Download -
September 15th, 2022NBE_9169_v114_revA_15Sep2022EnglishGridion Download -
September 15th, 2022NBE_9169_v114_revA_15Sep2022EnglishMinion Download -