3rd October 2025
- In 'Overview of the protocol', 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 4, 'Native barcode ligation', an important note was included to only use the barcode wells once and in steps 10, 11 and 13, text was removed for the clear cap EDTA (a reagent in the previous version of the SQK-NBD114.96 kit) as it is no longer applicable. And in step 16, the text was updated to resuspend the beads in SFB by flicking.
- In section 5, 'Adapter ligation and clean-up', 'Check your flow cell' and section 6, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 6, 'Priming and loading the MinION and GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction.
- In 'Troubleshooting', video links for the PromethION Flow Cell loading were included alongside the MinION Flow Cell loading.
10/8/2025
NBA_9170_v114_revR_03Oct2025
English
MinION
3rd October 2025
- In 'Overview of the protocol', 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 4, 'Native barcode ligation', an important note was included to only use the barcode wells once and in steps 10, 11 and 13, text was removed for the clear cap EDTA (a reagent in the previous version of the SQK-NBD114.96 kit) as it is no longer applicable. And in step 16, the text was updated to resuspend the beads in SFB by flicking.
- In section 5, 'Adapter ligation and clean-up', 'Check your flow cell' and section 6, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 6, 'Priming and loading the MinION and GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction.
- In 'Troubleshooting', video links for the PromethION Flow Cell loading were included alongside the MinION Flow Cell loading.
10/8/2025
NBA_9170_v114_revR_03Oct2025
English
PromethION
3rd October 2025
- In 'Overview of the protocol', 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 4, 'Native barcode ligation', an important note was included to only use the barcode wells once and in steps 10, 11 and 13, text was removed for the clear cap EDTA (a reagent in the previous version of the SQK-NBD114.96 kit) as it is no longer applicable. And in step 16, the text was updated to resuspend the beads in SFB by flicking.
- In section 5, 'Adapter ligation and clean-up', 'Check your flow cell' and section 6, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 6, 'Priming and loading the MinION and GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction.
- In 'Troubleshooting', video links for the PromethION Flow Cell loading were included alongside the MinION Flow Cell loading.
10/3/2025
NBA_9170_v114_revR_03Oct2025
English
GridION
3rd October 2025
- In 'Overview of the protocol', 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 4, 'Native barcode ligation', an important note was included to only use the barcode wells once and in steps 10, 11 and 13, text was removed for the clear cap EDTA (a reagent in the previous version of the SQK-NBD114.96 kit) as it is no longer applicable. And in step 16, the text was updated to resuspend the beads in SFB by flicking.
- In section 5, 'Adapter ligation and clean-up', 'Check your flow cell' and section 6, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 6, 'Priming and loading the MinION and GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction.
- In 'Troubleshooting', video links for the PromethION Flow Cell loading were included alongside the MinION Flow Cell loading.
10/3/2025
NBA_9170_v114_revR_03Oct2025
English
MinION
3rd October 2025
- In 'Overview of the protocol', 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 4, 'Native barcode ligation', an important note was included to only use the barcode wells once and in steps 10, 11 and 13, text was removed for the clear cap EDTA (a reagent in the previous version of the SQK-NBD114.96 kit) as it is no longer applicable. And in step 16, the text was updated to resuspend the beads in SFB by flicking.
- In section 5, 'Adapter ligation and clean-up', 'Check your flow cell' and section 6, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 6, 'Priming and loading the MinION and GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction.
- In 'Troubleshooting', video links for the PromethION Flow Cell loading were included alongside the MinION Flow Cell loading.
10/3/2025
NBA_9170_v114_revR_03Oct2025
English
PromethION
3rd October 2025
- In 'Overview of the protocol', 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 4, 'Native barcode ligation', an important note was included to only use the barcode wells once and in steps 10, 11 and 13, text was removed for the clear cap EDTA (a reagent in the previous version of the SQK-NBD114.96 kit) as it is no longer applicable. And in step 16, the text was updated to resuspend the beads in SFB by flicking.
- In section 5, 'Adapter ligation and clean-up', 'Check your flow cell' and section 6, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 6, 'Priming and loading the MinION and GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction.
- In 'Troubleshooting', video links for the PromethION Flow Cell loading were included alongside the MinION Flow Cell loading.
1/30/2025
NBA_9170_v114_revP_30Jan2025
English
GridION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
NBA_9170_v114_revP_30Jan2025
English
MinION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
NBA_9170_v114_revP_30Jan2025
English
PromethION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/12/2024
NBA_9170_v114_revO_12Dec2024
English
GridION
12th December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
NBA_9170_v114_revO_12Dec2024
English
MinION
12th December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
NBA_9170_v114_revO_12Dec2024
English
PromethION
12th December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
NBA_9170_v114_revM_12Dec2024
English
GridION
12th December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
NBA_9170_v114_revM_12Dec2024
English
MinION
12th December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
NBA_9170_v114_revM_12Dec2024
English
PromethION
12th December 2024
- Addition of links to MinION Mk1D IT requirements in "Computer requirements and software" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/2024
NBA_9170_v114_revN_15Sep2022
English
GridION
21st August 2024
- Removed Early access loading now that kit is in "released" phase.
- Removed reference to duplex sequencing.
- Updated flow cell loading recommendations based off input fragment size.
8/21/2024
NBA_9170_v114_revN_15Sep2022
English
MinION
21st August 2024
- Removed Early access loading now that kit is in "released" phase.
- Removed reference to duplex sequencing.
- Updated flow cell loading recommendations based off input fragment size.
8/21/2024
NBA_9170_v114_revN_15Sep2022
English
PromethION
21st August 2024
- Removed Early access loading now that kit is in "released" phase.
- Removed reference to duplex sequencing.
- Updated flow cell loading recommendations based off input fragment size.
21st September 2023
- Updated flow cell loading instructions to include light shield installation
- Format change in "Native barcode ligation" step to fix downloaded checklist.
21st September 2023
- Updated flow cell loading instructions to include light shield installation
- Format change in "Native barcode ligation" step to fix downloaded checklist.
21st September 2023
- Updated flow cell loading instructions to include light shield installation
- Format change in "Native barcode ligation" step to fix downloaded checklist.
14th August 2023
- Updated the equipment and consumables with both formats of kits which are being shipped.
- Updated the native barcoding ligation step to include instructions for both EDTA concentrations.
- Updated flow cell flushing instructions to include instructions for the bottle format kit.
14th August 2023
- Updated the equipment and consumables with both formats of kits which are being shipped.
- Updated the native barcoding ligation step to include instructions for both EDTA concentrations.
- Updated flow cell flushing instructions to include instructions for the bottle format kit.
14th August 2023
- Updated the equipment and consumables with both formats of kits which are being shipped.
- Updated the native barcoding ligation step to include instructions for both EDTA concentrations.
- Updated flow cell flushing instructions to include instructions for the bottle format kit.
March 2023
- Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents.
- Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 2023
- Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents.
- Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
March 2023
- Updated end-prep step to show the tip for making up an end-prep mastermix before combining the reagents.
- Corrected the expected volumes for pooled samples and the reflected in the AXP volumes.
18th October 2022
- Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
18th October 2022
- Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
18th October 2022
- Updated equipment and consumables to remove reference to the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing
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