ElysION user guide
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ElysION user guide V ELY_9212_v1_revA_30Oct2024
This is an Early Access device.
For more information about our Early Access programmes, please refer to this article on product release phases.
FOR RESEARCH USE ONLY
Contents
Overview
Installation
- 2. What's in the box
- 3. Connections and ports available on devices
- 4. Power on the ElysION robot
- 5. Power on the Compute Control Unit
- 6. Power off the devices
Software interface and use
- 7. Start the user interface and initialise
- 8. Check data storage capacity ahead of starting a run
- 9. Inserting a flow cell onto the ElysION device
- 10. Choose a method to run
- 11. Guide to setting the run variables
- 12. Guide to writing a sample sheet
- 13. Accessing the sequencing data
- 14. Accessing the EPI2ME™ output data
Device maintenance
Troubleshooting
Descripción general
This is an Early Access device.
For more information about our Early Access programmes, please refer to this article on product release phases.
1. Device Information
ElysION™
The ElysION™ is a fully automated sample-to-answer sequencing solution with an integrated MinION™ and onboard computing. It is designed to bring streamlined genomics into the lab by offering a range of end-to-end sequencing workflows for a broad field of research applications.
The ElysION device is a combined liquid handling and sequencing platform that is intended for use in a laboratory setting for the preparation and sequencing of nanopore libraries from a range of input materials.
This device is for research use only.
Current supported Oxford Nanopore Technologies' workflows:
- Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
- Native Barcoding Kit 96 V14 (SQK-NBD114.96)
- Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS)
- Rapid sequencing V14 - Plasmid sequencing
Current supported kits:
- Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
- Native Barcoding Kit 96 V14 (SQK-NBD114.96)
- Flow Cell Wash Kit XL (EXP-WSH004-XL)
Current supported flow cells:
- MinION Flow Cells R10.4.1 (FLO-MIN114)
2. What's in the box
What's in the box
Package includes:
- 1x ElysION Sample Preparation Device
- 1x ElysION Integration Kit - MinION
- 1x ElysION Control Unit - MinION
- 3x MinION Mk1D sequencing devices
- 3x MinION Flow Cells R10.4.1 (FLO-MIN114)
- 1x Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
- 1x License and Warranty (12 months)
Also included:
- Installation of instrument and software
- Installation qualification
- On-site training
3. Connections and ports available on devices
(A) ElysION robot
Attach the cables and peripherals to the ports shown in the image.
To connect a USB type keyboard and mouse or hard drive, connect a USB-C to USB-A multi-adaptor into the USB-C port.
We recommend this for accurate editing of sample sheets.
Connect the power cable to the ElysION and a mains supply.
(B) Compute Control Unit
Attach the cables and peripherals to the ports shown in the image.
Connect a keyboard and mouse to the USB type A ports.
Connect the computer to the internet with an Ethernet cable connection to Ethernet port 1.
Connect the computer to the ElysION robot with an Ethernet cable conection to Ethernet port 2.
Connect a display port cable between the computer and a monitor screen.
Connect the power cable to the computer and a mains supply.
4. Power on the ElysION robot
Switch on the ElysION by pressing the power button. The central light above the ElysION robot door should switch on.
The touch screen monitor attached to the ElysION will automatically power on.
If the touch screen monitor does not automatically switch on, press the monitor power button on the right-hand side of the underside of the monitor.
The Background Services (BotQL) will automatically start during power on.
You will have the option of interacting with ElysION by using either the touch screen monitor or a keyboard and mouse connected separately to this device. Please refer to connections and ports available on ElysION for more information on how to connect a keyboard and mouse.
5. Power on the Compute Control Unit
Turn on the Compute Control Unit by pressing the power button.
6. Power off the devices
Power off the ElysION.
You can turn off the ElysION robot using either of the following options:
- Press the Start icon at the bottom of the Windows menu on the touch screen monitor, then select Shut down.
or
- Press and hold the ElysION power button for five seconds.
Power off the Compute Control Unit.
Turn off the Compute Control Unit by clicking the power button in the top right corner and then clicking Power off.
7. Start the user interface and initialise
Before initialising the instrument, perform all daily maintenance steps and ensure that the worktable has been cleared of any labware or reagents and there are no obstructions.
For details of the daily maintenance, please refer to the maintenance schedule .
If you are unsure if the instrument is ready to initialise, please contact Customer Support.
Make sure the Background Services (BotQL) is running.
If not, double click the Background Services desktop application.
Note: The ElysION Background Services application needs to run simultaneously while running ElysION and cannot be shut down while the instrument is in use for either liquid handling, sequencing, or analysis.
Select the 'ElysION' application from the desktop of the ElysION robot touch screen monitor.
The window shown below will open.
Wait for the Liquid handler and MinION to connect to the Compute Control Unit software.
Once connected, the Liquid handler section will show an Initialise option and the MinION section will show that the MinION Mk1D is Available, as shown in the image below.
Ensure the worktable of the ElysION robot is cleared. Close the device door and press the 'Initialise' option.
FIN DEL PROCESO
Once initialisation has successfully completed, you will be able to run an automated method.
8. Check data storage capacity ahead of starting a run
Press 'System' and 'Device information' from the touch screen monitor.
Ensure there is sufficient storage space for your planned assay run.
We recommend a minimum of 30 Gbases of storage space for each run.
Oxford Nanopore Technologies can provide support to connect your instrument to an internal network to allow automated data offload from the ElysION device to your long-term storage. Please contact Customer Support.
9. Inserting a flow cell onto the ElysION device
Remove the MinION Mk1D flow cell cover.
Insert a MinION Flow Cell with all ports closed into the MinION Mk1D.
Press the flow cell down firmly into position.
Replace the MinION Mk1D flow cell cover.
10. Choose a method to run
In the ElysION application, press the 'Run method' tab on the left side of the window.
To begin a method, select from the list of available methods. Then press 'Next' at the bottom right corner of the screen.
Refer to the method specific user manual for further instructions on preparing your workflow.
11. Guide to setting the run variables
Types of run variables
During the preparation of the robot and ahead of starting a workflow, there are three optional variables that can be set.
(A) Sequencing time limit
This variable gives you the option to set the maximum length of time (in hours) the instrument will sequence for or the option to run until the flow cell pores are depleted (5% of total pore count). When either the time limit has been reached or the pores have been depleted, the instrument will stop sequencing and proceed to the next step in the workflow.
(B) Data target
This variable allows you to set a maximum sequencing data output (in Gbases). Once this data target has been achieved, the instrument will stop sequencing and proceed to the next step in the workflow. Alternatively, where no data target is set, sequencing will stop according to the set 'sequencing time limit' variable.
Note: Sequencing will stop when either the set sequencing time limit or set data target has been met. For example, if the sequencing time limit is set to 72 hours and the data target is set to 20 Gbases, and sequencing produces 20 Gbases in 48 hours, sequencing will stop before the 72 hours time limit.
(C) Flow cell flush/wash
This variable allows you to decide what happens to the flow cell once sequencing has completed. Flushing prepares the flow cell for return to Oxford Nanopore Technologies for recycling. Washing cleans the flow cell to allow multiple use of the same flow cell.
Note: Flush/Wash choices will require different reagents to be used. Washing will require an additional Oxford Nanopore Technologies' Flow Cell Wash Kit (EXP-WSH004).
12. Guide to writing a sample sheet
Writing a sample sheet to perform a workflow
All ElysION workflows require a sample sheet in order to perform a workflow. The sample sheet performs several functions:
- Instructs the instrument on how many samples to process.
- Instructs the instrument on which barcodes to attach to samples and to include in analysis.
Sample sheets contain unique parameter values required for the specific workflow being run. You can alter the parameters to ensure correct preparation of samples. If sample sheet parameters do not match the workflow assay or are in the incorrect format, the sample sheet will be rejected.
To avoid issues when writing a new sample sheet, we recommend you always design a new sample sheet from the template provided by Oxford Nanopore Technologies.
Values which must not be altered on the sample sheet (highlighted in pink):
Values which can be altered on the sample sheet (highlighted in green):
Rules of editing values on the sample sheet:
- library_id: any text to identify one library from another in a human readable format.
- created_by: any text to identify the user who wrote the sample sheet in a human readable format.
- created_at: the time stamp that the sample sheet file was created. This must be in the ISO 8601 format.
ISO 8601 formatting:
- 2024-06-21: The date (Year-Month-Day).
- T: The separator between the date and time (standard in ISO 8601).
- 14:07:14: The time in 24-hour format (Hours:Minutes:Seconds).
- +01:00: The time zone offset from UTC (in this case, it is 1 hour ahead of UTC).
sample_count: The exact number of samples being processed by the instrument given as an integer value. This value must be within the range possible for the workflow being prepared (see workflow specific documentation).
well_id: The id of the wells that the samples start in. This must always start at A1 and continue in a column wise list, with no gaps. The total number of well ids listed in the sample sheet must be equal to the sample count value also provided. Examples of correct and incorrect well_id configurations are shown in the Table:
Correct well_id configuration | Incorrect well_id configuration |
---|---|
A1, B1, C1, D1, E1, F1, G1, H1, A2 and sample count = 9 | B1, C1, D1, E1, F1, G1, H1, A2, B2 (A1 missing) |
A1, B1, C1, D1, F1, G1, H1, A2, B2 (E1 missing) | |
A1, A2, A3, A4, A5, A6, A7, A8 (Row format) | |
A1, B1, C1, D1, E1, F1, G1, H1 and sample count = 9 (only 8 samples listed) |
barcode: This list of barcode ids specifies which barcode from Oxford Nanopore Technologies' barcode plate is delivered to each sample (as specified in the well_id column). The first barcode can be taken from any position in the barcode plate however, all subsequent barcodes must follow in sequential order. The total number of barcode ids must match the total number of samples provided. Each barcode can only be used once and must be formatted as a two-digit number. Examples of correct and incorrect barcode configurations are shown in the Table:
Correct barcode configuration | Incorrect barcode configuration |
---|---|
barcode01, barcode02, barcode03, barcode04, barcode05, barcode06 and sample count = 6 | barcode03, barcode04, barcode05, barcode06, barcode01, barcode02 (not sequential) |
barcode06, barcode07, barcode08, barcode09, barcode10, barcode11, and sample count = 6 | barcode01, barcode02, barcode03, barcode04, barcode05, barcode08 (not sequential) |
barcode08, barcode09, barcode010, barcode11, barcode12, barcode13 (barcode010 has 3 digits) | |
barcode01, barcode02, barcode03, barcode04, barcode05, barcode06 and sample count = 8 (only 6 samples listed) |
sample_type: The sample type flag which is used during post-basecalling analysis in EPI2ME. Defaults as 'sample' if post-basecalling analysis is not required. If you plan to do analysis, see the workflow specific documentation.
sample_id: The sample id which is used during post-basecalling analysis in EPI2ME. Defaults as a unique integer if post-basecalling analysis is not required. If you plan to do analysis, see the workflow specific documentation.
13. Accessing the sequencing data
Log in on the Compute Control Unit monitor (separate from the ElysION touch screen monitor).
On the left-hand side of the screen, open the 'File manager' application.
Navigate to the 'data' folder.
Open 'output', then 'sequences', then 'no_group', then 'no_sample'.
This folder will display all the available sequencing run data within subfolders with the naming convention: 'YYYYMMDD_HHMM_sequencing device ID_Flow Cell ID_XXXXXXXX'.
We recommend to copy all sequencing data to a long-term data storage system regularly to prevent the Compute Unit storage from becoming full.
14. Accessing the EPI2ME™ output data
Log in on the Compute Control Unit monitor (separate from the ElysION touch screen monitor).
On the left-hand side of the screen, open the 'File manager' application.
Navigate to the 'data' folder.
Open 'reception', then 'instances'.
This folder will display all the available EPI2ME pipeline data within subfolders, e.g. 'wf-bacterial-genomes_01J6HWYJM7QSJDWV1SHVZEHSMZ'.
To identify the pipeline output which corresponds to an automated run, refer to the date edited on the right-hand side of the file manager screen.
Note: the date that a folder is edited/written as shown in the file manager will not be the same date as the start of the automated library prep. EPI2ME analysis will start once sequencing has finished. Therefore, the date-time written will depend on the date the automated prep was started and the length of time the sequencing was performed.
- We recommend to copy all EPI2ME data to a long-term data storage system regularly to prevent the Compute Unit storage from becoming full.
15. Cleaning the device
Maintenance and decontamination procedures
For full details of the maintenance and decontamination procedures, please refer to the 'ElysION operating manual' provided with your ElysION device.
Using chemical agents
The device should be cleaned with any of the listed agents and dried with a lint-free cloth. Use of any other chemical agents than those listed below or listed in the 'ElysION operating manual' may cause damage to the instrument or risk injury to you.
Cleaning agent | Specification |
---|---|
DI water | Distilled or deionized water |
Alcohol | 70% ethanol or 100% isopropanol (2‑propanol) |
Weak detergent | Liqui-Nox |
Disinfectant | Bacillol plus, Bomix, Lysetol FF |
System liquid | Distilled or deionized water |
Base | 0.025 to 0.25 mol/L sodium hydroxide |
Bleach | 6% sodium hypochlorite |
RNase AwayTM decontamination reagent | Alkali hydroxide <0.1% |
16. UV light disinfection
Select 'System' in the bottom left corner of the ElysION software, on the touch screen monitor, then 'Maintenance'.
Select 'UV light' from the list of maintenance methods.
Define the length of time for the UV disinfection and press 'Start'.
A cautionary message that the UV disinfection is running will appear with a time remaining report.
17. Maintenance schedule
ElysION maintenance schedule
Please refer to the 'ElysION operating manual' included with your ElysION device for details of the maintenance schedule.
Daily maintenance
- Empty both waste containers.
- Either decontaminate or replace with new waste containers.
Weekly maintenance
Clean the following with 70% ethanol:
- waste drawer
- DiTi drop station
- ElysION robot touch screen monitor
Monthly, quarterly, bi-annual, quinquennial maintenance
Please refer to the 'ElysION operating manual' for more information.
18. The ElysION robot does not switch on
Check that the ElysION touch screen monitor is switched on by pressing the monitor power button on the bottom right-hand side of the underside of the monitor.
Check that all power cables are attached correctly and are connected to a mains supply.
Press and hold the ElysION power button for one second.
19. Contacting Customer Support
IMPORTANTE
Contacting Customer Support
For all issues, contact newproductsupport@nanoporetech.com and include the device serial number in the description of your issue. Please refer to device serial number for more information.
For issues related to the running of automated methods, include the device serial number in the description of your issue and attach copies of your logging data. Please refer to how to collect logging data for troubleshooting support for more information.
20. Device serial number for troubleshooting support
Serial number for ElysION
The serial number for the ElysION can be found on the rear panel of the instrument. See images below:
Serial number for Compute Control Unit
The serial number for the Compute Control Unit can be found on the rear side of the unit, as shown in the image below:
21. How to collect logging data for troubleshooting support
On the touch screen monitor, run the 'Support' application from the desktop.
Copy the generated logging snapshot to a USB drive.
Send the snapshot file to Customer Support and include the serial number for your device in the description of your issue.
Please refer to device serial number for more information.