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Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) – automated ElysION (SQK-RBK114.96) (ISOE_9213_v114_revE_28Oct2025)


DateVersionLanguageDeviceChanges
10/28/2025ISOE_9213_v114_revE_28Oct2025EnglishMinION
28th October 2025 - In section 2, 'Equipment and consumables', text in the SQK-RBK114.96 kit image was edited. - In section 7, 'ElysION run setup', an important note was added to use the barcode wells only once. And a link was added to access an optional 'Pause for QC' step in the Appendix. - A new section 'NO-MISS Pause for QC' was created in the Appendix as an option for users to pause their run following automated DNA extraction in order to perform sample QC checks.
1/20/2025ISOE_9213_v114_revD_07Jan2025EnglishMinION
07 January 2025 - Updated ElysION consumable details with naming and product codes found in the Oxford Nanopore Technologies store.
1/7/2025ISOE_9213_v114_revD_07Jan2025EnglishMinION
07 January 2025 - Updated ElysION consumable details with naming and product codes found in the Oxford Nanopore Technologies store.
11/22/2024ISOE_9213_v114_revC_22Nov2024EnglishMinION
22 November 2024 - Updated nomenclatures in-line with marketing materials. - Updated "Sample preparation" section titles to more accurately represent the process carried out. - Updated title of section "Automated extraction, library preparation and sequencing" to more accurately represent the process carried out. - Removed reference to local distributors as these are currently not available.
11/8/2024ISOE_9213_v114_revB_09Nov2024EnglishMinION
08 November 2024 - Added instruction in the "ElysION run setup" to prepare the Rapid Adapter Master Mix by diluting Rapid Adapter (RA) in Adapter Buffer (ADB).
11/8/2024ISOE_9213_v114_revB_08Nov2024EnglishMinION
08 November 2024 - Added instruction in the "ElysION run setup" to prepare the Rapid Adapter Master Mix by diluting Rapid Adapter (RA) in Adapter Buffer (ADB).
10/30/2024ISOE_9213_v114_revA_30Oct2024EnglishMinION
Protocol release
10/28/2024ELYSION_NOMISS_v7EnglishMinION
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