2nd October 2025
- In 'Overview of the protocol', an important note on Kit14 sequencing and duplex basecalling info sheet was removed as this is a Legacy document and its information is no longer applicable.
- In 'Compatibility of this protocol', MinION Mk1B and Mk1C were replaced with Mk1D as these device documents have moved to legacy and their user manuals were removed from 'Data acquisition and basecalling'.
- - In 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. In 'Complete a flow cell check', the link for the flow cell check in MinKNOW was replaced with the flow cell check document. And an important note regarding the MinION Mk1D device leaving a small gap around the sides, when a flow cell is inserted and the lid is closed was added to the end of this section.
- In 'Priming and loading the PromethION Flow Cell', the step to remove the flow cell from the fridge was moved to the top of this section and a step to complete a flow cell check was included.
- In 'Data acquisition and basecalling' information on duplex basecalling was removed as it is no longer applicable.
10/2/2025
DTD_9181_v114_revL_02Oct2025
English
MinION
2nd October 2025
- In 'Overview of the protocol', an important note on Kit14 sequencing and duplex basecalling info sheet was removed as this is a Legacy document and its information is no longer applicable.
- In 'Compatibility of this protocol', MinION Mk1B and Mk1C were replaced with Mk1D as these device documents have moved to legacy and their user manuals were removed from 'Data acquisition and basecalling'.
- - In 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. In 'Complete a flow cell check', the link for the flow cell check in MinKNOW was replaced with the flow cell check document. And an important note regarding the MinION Mk1D device leaving a small gap around the sides, when a flow cell is inserted and the lid is closed was added to the end of this section.
- In 'Priming and loading the PromethION Flow Cell', the step to remove the flow cell from the fridge was moved to the top of this section and a step to complete a flow cell check was included.
- In 'Data acquisition and basecalling' information on duplex basecalling was removed as it is no longer applicable.
10/2/2025
DTD_9181_v114_revL_02Oct2025
English
PromethION
2nd October 2025
- In 'Overview of the protocol', an important note on Kit14 sequencing and duplex basecalling info sheet was removed as this is a Legacy document and its information is no longer applicable.
- In 'Compatibility of this protocol', MinION Mk1B and Mk1C were replaced with Mk1D as these device documents have moved to legacy and their user manuals were removed from 'Data acquisition and basecalling'.
- - In 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. In 'Complete a flow cell check', the link for the flow cell check in MinKNOW was replaced with the flow cell check document. And an important note regarding the MinION Mk1D device leaving a small gap around the sides, when a flow cell is inserted and the lid is closed was added to the end of this section.
- In 'Priming and loading the PromethION Flow Cell', the step to remove the flow cell from the fridge was moved to the top of this section and a step to complete a flow cell check was included.
- In 'Data acquisition and basecalling' information on duplex basecalling was removed as it is no longer applicable.
10/4/2023
DTD_9181_v114_revK_22Feb2023
English
GridION
4th October 2023
- Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
10/4/2023
DTD_9181_v114_revK_22Feb2023
English
MinION
4th October 2023
- Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
10/4/2023
DTD_9181_v114_revK_22Feb2023
English
PromethION
4th October 2023
- Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
ONT plc is a member of the producer compliance scheme run by ERP UK Ltd, who manage the submission of documentation in support of WEEE compliance for ONT plc’s manufacture and supply of Electrical and Electronic equipment in the UK.
