A guide to investigating methylation in the human genome
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Overview
Epigenetics is the study of chemical modifications that can alter phenotype without altering nucleotide sequence. The most well-characterised and widely studied modification in mammalian, including human genomes, is 5mC DNA methylation — the addition of a methyl group to a cytosine nucleotide.
In this getting started guide, discover how to sequence epigenetic modifications directly from native DNA molecules — in one go — without PCR or additional library preparation steps to reveal how methylation impacts phenotype, including its role in regulating gene expression.
In this getting started guide, you will:
- Find out how direct DNA Oxford Nanopore sequencing enhances the analysis of epigenetic modifications
- Discover the benefits of Oxford Nanopore sequencing
- Identify the appropriate Oxford Nanopore sequencing kit for your experiment
- For any questions, see the FAQs for this technique
- Read real-world case studies of how researchers are utilising Oxford Nanopore sequencing to understand aberrant methylation in gene promoters and its association with disease
