Please note: The data displayed below was generated using one of our legacy kits. Our current kit chemistry is still compatible with the outlined extraction kit.

Oxford Nanopore Technologies have tested the method linked below to extract plasmid DNA. The extracted samples were sequenced using the Plasmid Sequencing (using SQK-RBK004) protocol on MinION Flow Cells.

For our current method, please follow the Rapid sequencing V14 - Plasmid sequencing (SQK-RBK114.24 or SQK-RBK114.96) protocol.

• QIAGEN Plasmid Plus Midi Kit information and protocol

Below is an example of data generated from extracted plasmid DNA using the EPI2ME platform.

Figure 1. A summary table of the number of sequencing reads analysed per experimental barcode.

Figure 2. A figure of genome annotation for each plasmid assembled.

Version	Change
v4, 14th March 2025	Updated compatible kits list - removed discontinued and unsupported kits. Updated kit and protocol links to current chemistry versions.
v3, 14th August 2023	Updated URL links
v2, 11th July 2023	Removed reference to specific kit code
v1, 2nd March 2022	Initial document release