Cancer research
Getting started guides
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A guide to transcriptomics with Oxford Nanopore
This guide introduces cDNA and direct RNA Oxford Nanopore sequencing, for ultra-rich transcriptomic data without compromise.
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A guide to targeted sequencing with Oxford Nanopore
This guide provides an introduction to performing targeted nanopore sequencing.
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A guide to human genomics with Oxford Nanopore
This guide introduces how to sequence human genomes with Oxford Nanopore, from the construction of new, highly complete reference assemblies to the comprehensive identification of variants.
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A guide to investigating methylation in the human genome
This guide provides an introduction to the direct sequencing of DNA methylation in human genomes.
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A guide to single-cell transcriptomics with Oxford Nanopore
This guide introduces the single-cell RNA nanopore sequencing workflow, for ultra-rich data without compromise.
Most viewed
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Multiomic nanopore sequencing: revolutionising human disease research
Find out how multiomic nanopore sequencing can accelerate your disease research using a single platform.
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PromethION 24: sequencing to the power of 24
Multiply your insights with PromethION 24, the production-scale nanopore sequencer that puts you in control of up to 24 high-output flow cells. Discover more.
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PromethION 2 Integrated: small box, big picture
Explore the PromethION 2 Integrated and bring high-output Oxford Nanopore sequencing to your lab. Run up to two independent flow cells with onboard compute for real-time insights. Discover more.
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Accelerate your epigenetics research with Oxford Nanopore sequencing
In this flyer, find out how Oxford Nanopore sequencing uncovers the complete methylome and provides highly accurate and comprehensive base modification data for analysis.
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Hereditary Cancer Panel: targeted sequencing via adaptive sampling
In this flyer, discover how to utilise the Oxford Nanopore Hereditary Cancer Panel to investigate 258 key genes associated with hereditary cancer risk.
Workflow overviews
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Sequencing full-length transcripts for isoform-level expression analysis
This end-to-end workflow provides a flexible method to sequence full-length transcripts for isoform-level expression analysis.
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Comprehensive characterisation of cancer predisposition genes using the Hereditary Cancer Panel
Discover the Hereditary Cancer Panel workflow and streamline your precision oncology research.
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Characterising genomic and epigenomic variation between tumour-normal research samples using long nanopore sequencing reads
This end-to-end workflow overview provides a simple solution for detecting a wide range of tumour-specific variation in a single sequencing assay.
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Obtaining full-length isoforms from single cells with Oxford Nanopore sequencing
This end-to-end workflow provides a simple solution for single-cell transcriptome analysis from 10x Genomics cDNA with library preparation in approximately three hours.
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Comprehensive human genomic variant and methylation analysis with long Oxford Nanopore reads
This end-to-end workflow provides a scalable method to identify previously hidden and potentially pathogenic variants.
Featured resources
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Which library prep workflow is right for my experiment?
Whether you’re sequencing DNA, RNA, or cDNA, a single sample or many in multiplex, there’s an Oxford Nanopore library prep kit for you. In this masterclass, discover how to choose the right one for your workflow.
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Cell-free DNA sequencing and methylation detection — promising potential for non-invasive cancer monitoring
In this case study, find out how researchers are using nanopore technology to analyse cfDNA for cancer monitoring.
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New approaches for human and clinical research
Explore the advantages of Oxford Nanopore sequencing to detect genomic and epigenomic variants in human and clinical research.
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Accelerating cancer research through comprehensive genomic analysis
Unlock previously hidden variation with nanopore sequencing and characterise cancer at unprecedented resolution.
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From speed to scale: how do I choose the right human whole-genome sequencing workflow for my experiment?
In this masterclass, find the right human whole-genome sequencing workflow to meet your experimental goals. We’ll cover whole-genome analysis in just 24 hours through to scaling sequencing for large cohort research. In this masterclass, discover: • How Oxford Nanopore sequencing is advancing human g
Protocols
Analysis workflows
wf-somatic-variation
This workflow calls variants from the alignment files of a paired tumor/normal sample.
Latest research
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How ‘Sturgeon’ guides the surgeon in paediatric neuro-oncology
Authors: Mariska Sie, Oscar H.J. Eelkman Rooda, et al.
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Beyond bisulfite sequencing: resolving 5-hmC with nanopore sequencing unmasks the true-5mC methylation entropy landscape
Authors: Uri Bertocchi, Eyal Katz, Jonathan Jeffet, Assaf Grunwald, Neiv Gabay, Jasline Deek, Sujal Verma, Amit Shwartz, Gali Umschweif–Nevo, Bernard Lerer, Yael Roichman, Yuval Ebenstein
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Rapid and accurate pan-cancer subtyping using RNA-sequencing
Watch this on-demand webinar exploring how RNA sequencing is transforming childhood cancer diagnosis. Dr. Adam Shlien presents a multiscale transcriptomic atlas that matched or clarified diagnoses for 85% of paediatric tumours
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Rapid, comprehensive methylation-based classification of haematologic malignancies by nanopore sequencing
Authors: Tristan Achterberg, Carlo Vermeulen, Huub van der Ent, et al.
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It takes two — haplotype-specific identification of genetic and epigenetic variation using nanopore sequencing
Event overview Knowledge Exchanges provide the perfect opportunity to hear some of the very latest developments in nanopore sequencing from members of the Oxford Nanopore team. In this Knowledge Exchange, Philipp Rescheneder shares how the long-read capabilities of nanopore sequencing can be u
