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Highlights from London Calling

Discover how researchers are using Oxford Nanopore sequencing to generate new insights across diverse applications.

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Discover nanopore sequencing

  • What it can do
  • How it’s being used
  • How to get started

Latest protocols

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Telomere-to-telomere sequencing (T2T) from blood and cells using SQK-APK114, SQK-LSK114, and SQK-ULK114

This protocol describes an end-to-end workflow for telomere-to-telomere sequencing of human samples. For Research Use Only This is a registration-based Early Access product.
Workflow

Single-cell transcriptomics sequencing from 5’ cDNA prepared with 10x Genomics using SQK-LSK114

Learn how to sequence full‑length 5′ cDNA transcripts from 10x Genomics single‑cell assays using the Oxford Nanopore Ligation Sequencing Kit V14 (SQK‑LSK114). This high‑output method enables detailed isoform analysis, alternative splicing detection, SNP resolution, and single‑cell subtype identifica

Single-cell transcriptomics sequencing from 3’ cDNA prepared with 10x Genomics using SQK-LSK114 and EXP-PCA001

This is a single-cell transcriptomics method.
View all end-to-end workflows

Rapid sequencing DNA - PCR Barcoding Kit 24 V14 (SQK-RPB114.24)

This protocol: Uses genomic DNA Has a low input requirement Has a method involving tagmentation, barcoding and PCR amplification Allows multiplexing of 1–24 samples Is compatible with R10.4.1 flow cells For Research Use Only

Rapid Sequencing Kit V14 - gDNA (SQK-RAD114)

The fastest and simplest protocol for genomic DNA sequencing. For Research Use Only

Ligation sequencing DNA V14 (SQK-LSK114)

This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114). It is recommended that a Lambda control experiment is completed first to become familiar with the technology.
View all library prep protocols

Webinars and events

plasmid

Unlocking high-resolution, strain-resolved microbiome analyses | ASM Microbe 26

During this video we learn how full length Oxford Nanopore sequencing is unlocking high-resolution microbiome analyses

View recent webinars
mRNA

Oxford Nanopore at AMP 2026

Oxford Nanopore Technologies are exhibiting and presenting at AMP 2026 in November.

View upcoming events

White papers

Preview image for the plant, animal, and environmental sequencing white paper, 'Genomics for a changing planet'.White paper

Genomics for a changing planet: sequencing the living world

Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.

Clinical research white paper coverWhite paper

New approaches for human and clinical research

Explore the advantages of Oxford Nanopore sequencing to detect genomic and epigenomic variants in human and clinical research.

Case studies

Visualisation of an RNA strand with glowing orange sections in the backgroundCase study

Large-cohort cDNA sequencing advances multiomic insights into neurodegenerative disease

In this case study, discover how Kimberley Billingsley and her colleagues at the National Institutes of Health are using Oxford Nanopore large-cohort cDNA sequencing to research the human brain transcriptome, enhancing understanding of the drivers of neurodegenerative diseases.

Oxford Nanopore microbial profilingCase study

High-quality bacterial genomes without the complexity

In this case study, researchers show that near-complete bacterial genomes can be assembled using nanopore sequencing alone.

Getting started guides

Preview image for the bulk transcriptomics getting started guideGetting started guide

A guide to transcriptomics with Oxford Nanopore

This guide introduces cDNA and direct RNA Oxford Nanopore sequencing, for ultra-rich transcriptomic data without compromise.

Preview of the microbial getting sequencing getting started guideGetting started guide

A guide to microbial sequencing with Oxford Nanopore

A guide to get started with sequencing microbial samples with Oxford Nanopore.

Workflow overviews

Preview of the NO-MISS workflow overviewWorkflow overview

Nanopore-only microbial isolate sequencing solution (NO-MISS): flexible, rapid bacterial and fungal isolate whole-genome sequencing

This end-to-end workflow provides a rapid solution for whole-genome Oxford Nanopore sequencing of bacterial and fungal isolates.

Two-page image of the Hereditary Cancer Panel workflowWorkflow overview

Comprehensive characterisation of cancer predisposition genes using the Hereditary Cancer Panel

Discover the Hereditary Cancer Panel workflow and streamline your precision oncology research.

Brochures

Flyer for Oxford Nanopore Technologies' cDNA-PCR Sequencing Kits, featuring graphs, diagrams, and key product benefits for transcriptome analysis.Brochure

cDNA-PCR Sequencing Kits

In this flyer, discover how you can sequence full-length transcripts and annotate the transcriptome with confidence.

Flyer: Direct RNA Sequencing Kits — 2 pagesBrochure

Direct RNA Sequencing Kits

In this flyer, discover how you can sequence RNA in its native form. Plus, learn how the new Direct RNA Barcoding Kit unlocks multiplexed sequencing of up to 24 samples in a single run.

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