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Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.

In this beginner-friendly masterclass, discover how to analyse your Oxford Nanopore sequencing data. Find out how basecalling works, the common file formats you’ll encounter, and how to use MinKNOW to set up and monitor your sequencing run. Then, discover how to analyse your data with EPI2ME — no pr

Whether you’re sequencing DNA, RNA, or cDNA, a single sample or many in multiplex, there’s an Oxford Nanopore library prep kit for you. In this masterclass, discover how to choose the right one for your workflow. In this masterclass, discover: • The range of Oxford Nanopore library prep solutions av

Great sequencing results start with high-quality DNA and RNA extraction. In this masterclass, find out how to achieve just that — from choosing the right method for you to performing size selection, fragmentation, and sample handling. In this masterclass, discover: • How to optimise extraction for y

Your Oxford Nanopore sequencing journey starts here. In this masterclass, find out how nanopore sequencing works, what to expect at each step of your workflow, and how to plan your first experiment. In this masterclass, discover: • How Oxford Nanopore sequencing technology works and its benefits for

This protocol describes how to carry out native barcoding of genomic DNA (gDNA) using the Native Barcoding Kit 24 V14 (SQK-NBD114.24).

This document describes the barcoding of native genomic DNA libraries. For Research Use Only

This protocol describes how to carry out sequencing of a DNA sample using the Ligation Sequencing Kit V14 (SQK-LSK114). It is recommended that a Lambda control experiment is completed first to become familiar with the technology.

Transcriptome analysis of cDNA and direct RNA sequencing data.

Workflow for analysing Pore-c data for chromatin conformation capture.

The ONT Cas9 sequencing kit allows the enrichment of genomic regions of interest by amplifying target regions from adapters ligated to Cas9 cleavage sites. The purpose of this workflow is to assess the effectiveness of such Cas9 enrichment, but it can be applied to other enrichment approaches.

This workflow provides an easy way to align Oxford Nanopore reads and gather mapping stats either locally for small amounts of data or at scale in a distributed environment such as a cluster or the cloud.

Publication: DNA methylation reprogramming in marsupial embryos is restricted to the extraembryonic lineage

​Publication: Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa

Publication: Swine influenza-modified pulmonary microbiota

Problem: 16S and shotgun assembly and read-based approaches are widely used for taxonomic profiling. The resulted taxonomic assignments are compared across studies. We showed that the estimated taxa are biased by the choice of shotgun vs amplicon method. Methods: Target database: GTDB Re

Publication: A high-quality Oxford Nanopore assembly of the hourglass dolphin (Lagenorhynchus cruciger) genome