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Rapid sequencing DNA - 16S Barcoding Kit 24 V14 (SQK-16S114.24) (16S_9199_v114_revG_01Sep2025)

DateVersionLanguageDeviceChanges
9/1/202516S_9199_v114_revG_01Sep2025EnglishFlongle
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/1/202516S_9199_v114_revG_01Sep2025EnglishGridION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/1/202516S_9199_v114_revG_01Sep2025EnglishMinION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/1/202516S_9199_v114_revG_01Sep2025EspañolGridION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
9/1/202516S_9199_v114_revG_01Sep2025EspañolMinION
01 September 2025 - Included information on primer design and potential limitations to the Troubleshooting section of the protocol
8/26/202516S_9199_v114_revF_26Aug2025EnglishFlongle
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/26/202516S_9199_v114_revF_26Aug2025EnglishGridION
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/26/202516S_9199_v114_revF_26Aug2025EnglishMinION
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/26/202516S_9199_v114_revF_26Aug2025EspañolGridION
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/26/202516S_9199_v114_revF_26Aug2025EspañolMinION
26th August 2025 - Changed wording around the barcoding plates in the library preparation section of the protocol to align with both break-away and non-break-away plate formats.
8/21/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/202516S_9199_v114_revE_11Dec2024EspañolGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/202516S_9199_v114_revE_11Dec2024EspañolMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EspañolGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/22/202516S_9199_v114_revE_11Dec2024EspañolMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EspañolMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/14/202516S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/202416S_9199_v114_revE_11Dec2024EnglishFlongle
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/202416S_9199_v114_revE_11Dec2024EnglishGridION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/202416S_9199_v114_revE_11Dec2024EnglishMinION
11th December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
10/3/202416S_9199_v114_revD_03Oct2024EnglishFlongle
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_03Oct2024EnglishGridION
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_03Oct2024EnglishMinION
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_30Sept2024EnglishFlongle
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_30Sept2024EnglishGridION
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
10/3/202416S_9199_v114_revD_30Sept2024EnglishMinION
03rd October 2024 - Included specifications that a minimum of 4 samples / barcodes should be used for each library preparation.
9/30/202416S_9199_v114_revC_30Sept2024EnglishFlongle
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/202416S_9199_v114_revC_30Sept2024EnglishGridION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
9/30/202416S_9199_v114_revC_30Sept2024EnglishMinION
30th September 2024 - Moved protocol out of early access phase, removed early access wording.
4/24/202416S_9199_v114_revB_06Dec2023EnglishFlongle
24 April 2024 - Updated introduction to no longer reference 18S or ITS data. Workflow is mainly applicable to 16S data. - Updated nomenclature for EPI2ME (no longer EPI2ME labs).
4/24/202416S_9199_v114_revB_06Dec2023EnglishGridION
24 April 2024 - Updated introduction to no longer reference 18S or ITS data. Workflow is mainly applicable to 16S data. - Updated nomenclature for EPI2ME (no longer EPI2ME labs).
4/24/202416S_9199_v114_revB_06Dec2023EnglishMinION
24 April 2024 - Updated introduction to no longer reference 18S or ITS data. Workflow is mainly applicable to 16S data. - Updated nomenclature for EPI2ME (no longer EPI2ME labs).
April 24th, 202416S_9199_v114_revA_06Dec2023EnglishFlongle Download -
April 24th, 202416S_9199_v114_revA_06Dec2023EnglishGridion Download -
April 24th, 202416S_9199_v114_revA_06Dec2023EnglishMinion Download -

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