Human variation sequencing from blood samples using SQK-LSK114 (HVB_9206_v114_revH_22Jan2025)
| Date | Version | Language | Device | Changes | |
|---|---|---|---|---|---|
| 1/22/2025 | HVB_9206_v114_revH_22Jan2025 | English | PromethION | 22 January 2025 - Document title change - Extraction: Minor adjustments made to mitigate steps where errors may occur. - Size selection using SFE: Updated method in-line with latest recommendations. - Megaruptor shearing: Added more context for handling the device effectively. - Flow cell wash and reload: updated data images and included image for sequencing read length profile. | |
| 9/30/2024 | HVB_9206_v114_revG_14May2024 | English | PromethION | 21st August 2024 - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Data acquisition and basecalling instructions updated. | |
| 8/21/2024 | HVB_9206_v114_revG_14May2024 | English | PromethION | 21st August 2024 - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Data acquisition and basecalling instructions updated. | |
| August 21st, 2024 | HVB_9206_v114_revF_31Jul2024 | English | - | Download | 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 26 June 2024 - Swapped the use of Short Fragment Buffer (SFB) to Long Fragment Buffer (LFB) in the "Adapter ligation and clean-up" step of the protocol. |
| July 31st, 2024 | HVB_9206_v114_revE_14May2024 | English | - | Download | 26 June 2024 - Swapped the use of Short Fragment Buffer (SFB) to Long Fragment Buffer (LFB) in the "Adapter ligation and clean-up" step of the protocol. |
| June 26th, 2024 | HVB_9206_v114_revD_14May2024 | English | - | Download | 30 May 2024 - Corrected typo in introduction section. |
| May 30th, 2024 | HVB_9206_v114_revC_14May2024 | English | - | Download | 28 May 2024 - Corrected typo in DNA repair and end-prep for incubation following addition of NEBNext FFPE DNA reagents. |
| May 28th, 2024 | HVB_9206_v114_revB_14May2024 | English | - | Download | - |
| May 22nd, 2024 | HVB_9206_v114_revA_14May2024 | English | - | Download | - |