Ligation sequencing DNA XL V14 (SQK-LSK114-XL) (GDX_9163_v114_revT_30Jan2025)

DateVersionLanguageDeviceChanges
1/30/2025GDX_9163_v114_revT_30Jan2025EnglishGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDX_9163_v114_revT_30Jan2025EnglishMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025GDX_9163_v114_revT_30Jan2025EnglishPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/12/2024GDX_9163_v114_revS_12Dec2024EnglishGridION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024GDX_9163_v114_revS_12Dec2024EnglishMinION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024GDX_9163_v114_revS_12Dec2024EnglishPromethION
12 December 2024 - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/2024GDX_9163_v114_revR_29Jun2022EnglishGridION
21st August 2024 - Updated protocol introduction to trimmed down format. - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDX_9163_v114_revR_29Jun2022EnglishMinION
21st August 2024 - Updated protocol introduction to trimmed down format. - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024GDX_9163_v114_revR_29Jun2022EnglishPromethION
21st August 2024 - Updated protocol introduction to trimmed down format. - Updated sample input recommendations with table for different DNA size. - NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB, E7672S or E7672L) is now the recommended reagent module. - Updated the Repair and End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
August 21st, 2024GDX_9163_v114_revQ_31Jul2024EnglishMinion Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
August 21st, 2024GDX_9163_v114_revQ_31Jul2024EnglishPromethion Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
August 21st, 2024GDX_9163_v114_revQ_31Jul2024EnglishGridion Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 4th October 2023 - Updated the format of the final step in the "Adapter ligation and clean-up" step for the checklist
October 4th, 2023GDX_9163_v114_revO_29Jun2022EnglishMinion Download 27th September 2023 - Correct error in an equipment and consumable snippet regarding input recommendation for the library preparation
October 4th, 2023GDX_9163_v114_revO_29Jun2022EnglishPromethion Download 27th September 2023 - Correct error in an equipment and consumable snippet regarding input recommendation for the library preparation
October 4th, 2023GDX_9163_v114_revO_29Jun2022EnglishGridion Download 27th September 2023 - Correct error in an equipment and consumable snippet regarding input recommendation for the library preparation
September 27th, 2023GDX_9163_v114_revN_29Jun2022EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for high molecular weight DNA
September 27th, 2023GDX_9163_v114_revN_29Jun2022EnglishPromethion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for high molecular weight DNA
September 27th, 2023GDX_9163_v114_revN_29Jun2022EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step. - Update the equipment and consumables section to only be relevant for high molecular weight DNA
September 19th, 2023GDX_9163_v114_revM_29Jun2022EnglishMinion Download May 2023: - Updated the elution incubation step in the "DNA and end-prep" step of the protocol to be uniform with the SQK-LSK114 protocols. - This kit has moved to released phase.
September 19th, 2023GDX_9163_v114_revM_29Jun2022EnglishPromethion Download May 2023: - Updated the elution incubation step in the "DNA and end-prep" step of the protocol to be uniform with the SQK-LSK114 protocols. - This kit has moved to released phase.
September 19th, 2023GDX_9163_v114_revM_29Jun2022EnglishGridion Download May 2023: - Updated the elution incubation step in the "DNA and end-prep" step of the protocol to be uniform with the SQK-LSK114 protocols. - This kit has moved to released phase.
April 3rd, 2023GDX_9163_v114_revL_29Jun2022EnglishMinion Download April 2023: - Updated the elution incubation step in the "DNA and end-prep" step of the protocol to be uniform with the SQK-LSK114 protocols.
April 3rd, 2023GDX_9163_v114_revL_29Jun2022EnglishPromethion Download April 2023: - Updated the elution incubation step in the "DNA and end-prep" step of the protocol to be uniform with the SQK-LSK114 protocols.
April 3rd, 2023GDX_9163_v114_revL_29Jun2022EnglishGridion Download April 2023: - Updated the elution incubation step in the "DNA and end-prep" step of the protocol to be uniform with the SQK-LSK114 protocols.
March 15th, 2023GDX_9163_v114_revK_29Jun2022EnglishMinion Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
March 15th, 2023GDX_9163_v114_revK_29Jun2022EnglishPromethion Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
March 15th, 2023GDX_9163_v114_revK_29Jun2022EnglishGridion Download March 2023: - Updated the "Sequencing and data analysis" section to include further details about duplex basecalling
March 15th, 2023GDX_9163_v114_revJ_29Jun2022EnglishMinion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
March 15th, 2023GDX_9163_v114_revJ_29Jun2022EnglishPromethion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
March 15th, 2023GDX_9163_v114_revJ_29Jun2022EnglishGridion Download January 2023 - Changed the "Priming and loading the PrometION flow cell" section to the updated version.
October 27th, 2022GDX_9163_v114_revI_29Jun2022EnglishMinion Download 27th October 2022 - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 27th, 2022GDX_9163_v114_revI_29Jun2022EnglishPromethion Download 27th October 2022 - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 27th, 2022GDX_9163_v114_revI_29Jun2022EnglishGridion Download 27th October 2022 - Updated protocol to use 80% ethanol rather than 70% ethanol.
October 4th, 2022GDX_9163_v114_revH_29Jun2022EnglishMinion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022GDX_9163_v114_revH_29Jun2022EnglishPromethion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
October 4th, 2022GDX_9163_v114_revH_29Jun2022EnglishGridion Download 4th Oct 2022 - Updated kit 14 info sheet snippet and link
August 18th, 2022GDX_9163_v114_revG_29Jun2022EnglishMinion Download 18th August 2022 - Updated kit contents image
August 18th, 2022GDX_9163_v114_revG_29Jun2022EnglishPromethion Download 18th August 2022 - Updated kit contents image
August 18th, 2022GDX_9163_v114_revG_29Jun2022EnglishGridion Download 18th August 2022 - Updated kit contents image
August 10th, 2022GDX_9163_v114_revF_29Jun2022EnglishMinion Download 10th August 2022 - Added specific concentration of BSA required for preparing the flow cell priming mix
August 10th, 2022GDX_9163_v114_revF_29Jun2022EnglishPromethion Download 10th August 2022 - Added specific concentration of BSA required for preparing the flow cell priming mix
August 10th, 2022GDX_9163_v114_revF_29Jun2022EnglishGridion Download 10th August 2022 - Added specific concentration of BSA required for preparing the flow cell priming mix
August 10th, 2022GDX_9163_v114_revE_29Jun2022EnglishMinion Download 10th August 2022 Updated phrasing used to add BSA to the flow cell priming mix and the use of ligation buffer
August 10th, 2022GDX_9163_v114_revE_29Jun2022EnglishPromethion Download 10th August 2022 Updated phrasing used to add BSA to the flow cell priming mix and the use of ligation buffer
August 10th, 2022GDX_9163_v114_revE_29Jun2022EnglishGridion Download 10th August 2022 Updated phrasing used to add BSA to the flow cell priming mix and the use of ligation buffer
August 10th, 2022GDX_9163_v114_revD_29Jun2022EnglishMinion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
August 10th, 2022GDX_9163_v114_revD_29Jun2022EnglishPromethion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
August 10th, 2022GDX_9163_v114_revD_29Jun2022EnglishGridion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
August 10th, 2022GDX_9163_v114_revD_29Jun2022EnglishMinion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
August 10th, 2022GDX_9163_v114_revD_29Jun2022EnglishPromethion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
August 10th, 2022GDX_9163_v114_revD_29Jun2022EnglishGridion Download 9th August 2022 - "Priming and loading the SpotON flow cell" has been updated to include the use of BSA for MinION and GridION flow cells
July 21st, 2022GDX_9163_v114_revC_29Jun2022EnglishMinion Download 21st July 2022 - Updated the flow cell loading recommendations for MinION/GridION and PromethION flow cells
July 21st, 2022GDX_9163_v114_revC_29Jun2022EnglishPromethion Download 21st July 2022 - Updated the flow cell loading recommendations for MinION/GridION and PromethION flow cells
July 21st, 2022GDX_9163_v114_revC_29Jun2022EnglishGridion Download 21st July 2022 - Updated the flow cell loading recommendations for MinION/GridION and PromethION flow cells
July 21st, 2022GDX_9163_v114_revB_29Jun2022EnglishMinion Download 21st July 2022 - Updated flow cell loading recommendations for both MinION/GridION and PromethION - Edited protocol to be inclusive of both gDNA and amplicon DNA inputs: - Overview edited to reflect change above. - Included "Category" and "Sample type" to reflect change above - Included snippet in "Equipment and consumables" and "DNA repair and end- prep" to clarify difference in DNA sample inputs. - Have made "Materials" more inclusive to both DNA input types. - All relevant snippets should now account for both sample input types.
July 21st, 2022GDX_9163_v114_revB_29Jun2022EnglishPromethion Download 21st July 2022 - Updated flow cell loading recommendations for both MinION/GridION and PromethION - Edited protocol to be inclusive of both gDNA and amplicon DNA inputs: - Overview edited to reflect change above. - Included "Category" and "Sample type" to reflect change above - Included snippet in "Equipment and consumables" and "DNA repair and end- prep" to clarify difference in DNA sample inputs. - Have made "Materials" more inclusive to both DNA input types. - All relevant snippets should now account for both sample input types.
July 21st, 2022GDX_9163_v114_revB_29Jun2022EnglishGridion Download 21st July 2022 - Updated flow cell loading recommendations for both MinION/GridION and PromethION - Edited protocol to be inclusive of both gDNA and amplicon DNA inputs: - Overview edited to reflect change above. - Included "Category" and "Sample type" to reflect change above - Included snippet in "Equipment and consumables" and "DNA repair and end- prep" to clarify difference in DNA sample inputs. - Have made "Materials" more inclusive to both DNA input types. - All relevant snippets should now account for both sample input types.
June 30th, 2022GDX_9163_v114_revA_29Jun2022EnglishMinion Download 23rd June 2022 -Finalised full protocol for the Kit 14 contents. Just missing image in the Ligation Sequencing Kit XL (SQK-LSK114-XL) contents 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
June 30th, 2022GDX_9163_v114_revA_29Jun2022EnglishPromethion Download 23rd June 2022 -Finalised full protocol for the Kit 14 contents. Just missing image in the Ligation Sequencing Kit XL (SQK-LSK114-XL) contents 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
June 30th, 2022GDX_9163_v114_revA_29Jun2022EnglishGridion Download 23rd June 2022 -Finalised full protocol for the Kit 14 contents. Just missing image in the Ligation Sequencing Kit XL (SQK-LSK114-XL) contents 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
June 29th, 2022Rev1EnglishMinion Download 23rd June 2022 -Finalised full protocol for the Kit 14 contents. Just missing image in the Ligation Sequencing Kit XL (SQK-LSK114-XL) contents 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
June 29th, 2022Rev1EnglishPromethion Download 23rd June 2022 -Finalised full protocol for the Kit 14 contents. Just missing image in the Ligation Sequencing Kit XL (SQK-LSK114-XL) contents 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
June 29th, 2022Rev1EnglishGridion Download 23rd June 2022 -Finalised full protocol for the Kit 14 contents. Just missing image in the Ligation Sequencing Kit XL (SQK-LSK114-XL) contents 20th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.