Direct RNA sequencing (SQK-RNA004) (DRS_9195_v4_revF_11Dec2024)

DateVersionLanguageDeviceChanges
1/30/2025DRS_9195_v4_revF_11Dec2024EnglishGridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/30/2025DRS_9195_v4_revF_11Dec2024EnglishMinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/30/2025DRS_9195_v4_revF_11Dec2024EnglishPromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/30/2025DRS_9195_v4_revF_11Dec2024EspañolGridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/30/2025DRS_9195_v4_revF_11Dec2024EspañolMinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/30/2025DRS_9195_v4_revF_11Dec2024EspañolPromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/30/2025DRS_9195_v4_revF_11Dec2024中文GridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/30/2025DRS_9195_v4_revF_11Dec2024中文MinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/30/2025DRS_9195_v4_revF_11Dec2024中文PromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/27/2025DRS_9195_v4_revF_11Dec2024EnglishGridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/27/2025DRS_9195_v4_revF_11Dec2024EnglishMinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/27/2025DRS_9195_v4_revF_11Dec2024EnglishPromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/27/2025DRS_9195_v4_revF_11Dec2024EspañolGridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/27/2025DRS_9195_v4_revF_11Dec2024EspañolMinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/27/2025DRS_9195_v4_revF_11Dec2024EspañolPromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/27/2025DRS_9195_v4_revF_11Dec2024中文GridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/27/2025DRS_9195_v4_revF_11Dec2024中文MinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
1/27/2025DRS_9195_v4_revF_11Dec2024中文PromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/14/2024DRS_9195_v4_revF_11Dec2024EnglishGridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/14/2024DRS_9195_v4_revF_11Dec2024EnglishMinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/14/2024DRS_9195_v4_revF_11Dec2024EnglishPromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/14/2024DRS_9195_v4_revF_11Dec2024EspañolGridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/14/2024DRS_9195_v4_revF_11Dec2024EspañolMinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/14/2024DRS_9195_v4_revF_11Dec2024EspañolPromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/14/2024DRS_9195_v4_revF_11Dec2024中文GridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/14/2024DRS_9195_v4_revF_11Dec2024中文MinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/14/2024DRS_9195_v4_revF_11Dec2024中文PromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DRS_9195_v4_revF_11Dec2024EnglishGridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DRS_9195_v4_revF_11Dec2024EnglishMinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DRS_9195_v4_revF_11Dec2024EnglishPromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DRS_9195_v4_revF_11Dec2024EspañolGridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DRS_9195_v4_revF_11Dec2024EspañolMinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DRS_9195_v4_revF_11Dec2024EspañolPromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DRS_9195_v4_revF_11Dec2024中文GridION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DRS_9195_v4_revF_11Dec2024中文MinION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024DRS_9195_v4_revF_11Dec2024中文PromethION
11th Dec 2024 - Updated kit content to include information on different RNA CS (RCS) vial concentration in new kit batches. - Updated instructions to include options for lower RNA CS (RCS) concentration (old) and higher RNA CS (RCS) concentraction (new). - Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
10/16/2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EspañolGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EspañolMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EspañolPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EspañolGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EspañolMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EspañolPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/16/2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/14/2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/14/2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/14/2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/14/2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/14/2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/14/2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
10/11/2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
9/23/2024DRS_9195_v4_revE_23Sep2024EnglishGridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
9/23/2024DRS_9195_v4_revE_23Sep2024EnglishMinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
9/23/2024DRS_9195_v4_revE_23Sep2024EnglishPromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
9/23/2024DRS_9195_v4_revE_23Sep2024中文GridION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
9/23/2024DRS_9195_v4_revE_23Sep2024中文MinION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
9/23/2024DRS_9195_v4_revE_23Sep2024中文PromethION
23 Sept 2024 - Updated step 18 in Library preparation with clearer wording for the method. - Updated name of the step "Priming and loading the SpotON flow cell" to "Priming and loading the MinION and GridION flow cell".
8/27/2024DRS_9195_v4_revD_20Sep2023EnglishGridION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
8/27/2024DRS_9195_v4_revD_20Sep2023EnglishMinION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
8/27/2024DRS_9195_v4_revD_20Sep2023EnglishPromethION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
8/27/2024DRS_9195_v4_revD_20Sep2023中文GridION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
8/27/2024DRS_9195_v4_revD_20Sep2023中文MinION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
8/27/2024DRS_9195_v4_revD_20Sep2023中文PromethION
31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
August 12th, 2024DRS_9195_v4_revC_20Sep2023EnglishPromethion Download 31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
August 12th, 2024DRS_9195_v4_revC_20Sep2023EnglishGridion Download 31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
August 12th, 2024DRS_9195_v4_revC_20Sep2023EnglishMinion Download 31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells.
November 15th, 2023DRS_9195_v4_revB_20Sep2023EnglishPromethion Download -
November 15th, 2023DRS_9195_v4_revB_20Sep2023EnglishGridion Download -
November 15th, 2023DRS_9195_v4_revB_20Sep2023EnglishMinion Download -