Sequencing full-length transcripts for isoform-level expression analysis

Overview

Accurate quantification and characterisation of isoform expression is important to investigate the mechanisms of diseases. Nanopore sequencing can span full-length transcripts in single reads, enabling comprehensive transcriptome characterisation at the isoform level, which simplifies transcriptome annotation to reveal novel transcripts and isoforms. This end-to-end workflow provides a flexible method to sequence full-length transcripts for isoform-level expression analysis.

In this workflow, you will:

• Find out how full-length transcript sequencing enhances bulk transcriptomics
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices