Sequencing full-length transcripts for isoform-level expression analysis
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Overview
Accurate quantification and characterisation of isoform expression is important to investigate the mechanisms of diseases. Nanopore sequencing can span full-length transcripts in single reads, enabling comprehensive transcriptome characterisation at the isoform level, which simplifies transcriptome annotation to reveal novel transcripts and isoforms.
This end-to-end workflow provides a flexible method to sequence full-length transcripts for isoform-level expression analysis.
In this workflow overview, you will:
- Find out how full-length transcript sequencing enhances bulk transcriptomics
- Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
- Learn about our recommended sequencing kit and devices