Document repository

267 Results

Sort by

Ultra-rapid singleplex Adaptive Sampling

This protocol describes an ultra-rapid library preparation and sequencing of one single sample per PromethION Flow Cell, using the Ligation Sequencing Kit and the Adaptive Sampling target enrichment function ...

November 11, 2026

Singleplex Adaptive Sampling

This protocol describes a sequencing method for high-depth targeted sequencing of tumour, blood or bone marrow samples, enabling methylation analysis and comprehensive somatic variant detection via Adaptive Sampling.

November 11, 2026

Multiplex methylation whole genome sequencing

This protocol describes a nanopore sequencing method for low-pass whole genome sequencing to enable methylation-based classification of four tumour samples per PromethION Flow Cell.

November 11, 2026

Multiplex Adaptive Sampling

This protocol describes the process for library preparation and Adaptive Sampling seqeuncing for three samples per PromethION Flow Cell, using the Native Barcoding Kit and the Adaptive Sampling target enrichment ...

November 11, 2026

Genomic DNA extraction from human blood using Qiagen PureGene kit

This protocol describes a method to extract genomic DNA from human blood samples using the Puregene Blood Kit from Qiagen.

November 11, 2026

High Throughput Barcoding sequencing (SQK-HTB114.96) on PromethION Plus Flow Cells (FLO-PRO114P)

September 9, 2026

Rapid PCR barcoding DNA V14 – automated ElysION MinION (SQK-RPB114.24)

This is an automated rapid PCR barcoding method using the ElysION™ device with a MinION™ setup, outlining library preparation and sequencing.

August 26, 2026

Rapid barcoding DNA V14 – automated ElysION MinION (SQK-RBK114.96)

This is an automated rapid barcoding method using the ElysION™ device with a MinION™ setup, outlining library preparation and sequencing.

August 26, 2026

Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) – automated ElysION MinION (SQK-RBK114.96)

This is an automated end-to-end method using the ElysION™ device with a MinION™ setup, outlining sample extraction, library preparation, sequencing, and analysis.

August 26, 2026

Native barcoding DNA V14 – automated ElysION MinION (SQK-NBD114.96)

This is an automated native barcoding method using the ElysION™ device with a MinION™ setup, outlining library preparation and sequencing.

August 26, 2026

Microbial amplicon barcoding for 16S and ITS – automated ElysION MinION (SQK-MAB114.24)

This is an automated microbial amplicon barcoding method using the ElysION™ device with a MinION™ setup, outlining library preparation and sequencing.

August 26, 2026

Long read de novo sequencing from blood using SQK-LSK114 – scalable (near) telomere-to-telomere method

This protocol describes an end-to-end workflow for long read de novo sequencing of human samples. For Research Use Only

July 29, 2026

Long read de novo sequencing from blood and cells using SQK-ULK114 – expert telomere-to-telomere method

This protocol describes an end-to-end workflow for long read de novo sequencing of human samples. For Research Use Only

July 29, 2026

High Throughput Barcoding sequencing (SQK-HTB114.96)

June 10, 2026

Analysis of scalable DNA extraction using the Promega Maxwell RSC for human blood, saliva and buccal swab processing

In this study, we evaluated the Maxwell RSC extraction platform from Promega for its compatibility with Oxford Nanopore Technologies sequencing.

June 10, 2026

Direct RNA barcoding (SQK-DRB004.24)

March 30, 2026

SARS-CoV-2 virus PCR tiling and sequencing from RNA using SQK-RBK114.96

This protocol is for the extraction and sequencing of ultra-high molecular weight (uHMW) genomic DNA.

December 8, 2025

Human variation sequencing from 15kb extracted cell line samples using SQK-LSK114

This protocol describes an end-to-end process to prepare and sequence gDNA from cultured cell samples and analyse them using the “Human variant workflow” in EPI2ME.

October 24, 2025

Human variation sequencing from 15kb extracted blood samples using SQK-LSK114

This is an end-to-end method outlining sample extraction, library preparation, sequencing and data analysis. For Research Use Only Document version: HVSB_9230_v114_revA_24Oct2025

October 24, 2025

Automated and scalable DNA shearing with the Hamilton STAR for Oxford Nanopore Technologies sequencing

A high-throughput method to mechanically shear gDNA directly on the deck of the Hamilton Star, using a “tip-shearing” approach.

September 25, 2025

Document repository

Ultra-rapid singleplex Adaptive Sampling

Singleplex Adaptive Sampling

Multiplex methylation whole genome sequencing

Multiplex Adaptive Sampling

Genomic DNA extraction from human blood using Qiagen PureGene kit

High Throughput Barcoding sequencing (SQK-HTB114.96) on PromethION Plus Flow Cells (FLO-PRO114P)

Rapid PCR barcoding DNA V14 – automated ElysION MinION (SQK-RPB114.24)

Rapid barcoding DNA V14 – automated ElysION MinION (SQK-RBK114.96)

Nanopore-only Microbial Isolate Sequencing Solution (NO-MISS) – automated ElysION MinION (SQK-RBK114.96)

Native barcoding DNA V14 – automated ElysION MinION (SQK-NBD114.96)

Microbial amplicon barcoding for 16S and ITS – automated ElysION MinION (SQK-MAB114.24)

Long read de novo sequencing from blood using SQK-LSK114 – scalable (near) telomere-to-telomere method

Long read de novo sequencing from blood and cells using SQK-ULK114 – expert telomere-to-telomere method

High Throughput Barcoding sequencing (SQK-HTB114.96)

Analysis of scalable DNA extraction using the Promega Maxwell RSC for human blood, saliva and buccal swab processing

Direct RNA barcoding (SQK-DRB004.24)

SARS-CoV-2 virus PCR tiling and sequencing from RNA using SQK-RBK114.96

Human variation sequencing from 15kb extracted cell line samples using SQK-LSK114

Human variation sequencing from 15kb extracted blood samples using SQK-LSK114

Automated and scalable DNA shearing with the Hamilton STAR for Oxford Nanopore Technologies sequencing

Getting started

Quick links

About Oxford Nanopore