Device part number

MIN-101D

Device name

MinION Mk1D

Product overview

The MinION™ Mk1D is the next generation of portable nanopore sequencing devices. It has been designed to be compatible with modern laptops, computers and the iPad Pro (in combination with the MinION Mk1D iPad Accessory when released) via its USB-C connectivity.

Improved thermal dissipation capabilities of the MinION Mk1D significantly enhances sequencing performance, enabling highly accurate real-time sequencing in an even wider range of environments than its predecessor, the MinION Mk1B. The MinION Mk1D also has intuitive status LEDs to enable you to identify the status of the device at a glance.

The MinION Mk1D can be used with MinION Flow Cells or Flongle™ Flow Cells with the use of the Flongle adapter. The MinION Mk1D device can be used for both DNA and RNA sequencing and is operated using the MinKNOW™ software, which controls the device and flow cells, collects the sequencing data and performs basecalling.

Technical specifications

Component	Specification
Size (H x W x D) (mm)	13 x 55 x 125
Weight (g)	130
Maximum rated power (W)	7.5
Installation ports	1 x USB-C
Software installed	MinION driver
Sequencing temperature	Designed to sequence at environmental temperatures of +10°C to +35°C*
Installation requirement	Maintain 5 cm clearance to all but the bottom face of the device. Do not place the device on top of a laptop/computer.

*Functional range of electronics is within environmental temperatures of +5°C to +40°C

Shipping and logistics

The Oxford Nanopore Technologies MinION device is stored and shipped at ambient temperature (+2°C to +25°C). MinION devices are shipped either in a padded envelope or a shipping box with flow cells and reagents.

Please note that the MinION is shipped separately to the kits and flow cells in the Starter Pack.

IT requirements

MinION Mk1D IT requirements

Important - Only USB-C ports are supported to connect your MinION Mk1D to your computer. Ensure your computer has a USB-C port available. USB-A to USB-C adapters or USB-A to USB-C cables are not supported. USB-A ports typically cannot deliver enough power for the MinION Mk1D and may compromise sequencing performance.

Safety and legal information

Oxford Nanopore Technologies MinION Mk1D device is an electronic analysis system for use in scientific research.

This product is for research use only.

The safety information below provides you with the details needed to install and use the system safely.

Emergency procedures

In case of an emergency, switch the computer off at the power switch and unplug the USB-C cable.

Declaration of conformity

The MinION Mk1D conforms to the EMC and RoHS directives as outlined in the EU Declaration of Conformity.

International standards

The MinION Mk1D is certified to the following international standards:

Certification	Country
MET; UL61010/CSA-C22.2 No. 61010, third Edition: Electrical Equipment for Measurement, Control and Laboratory Use, Revision: May 11, 2012	USA and Canada
FCC	USA
RCM compliance	Australia and New Zealand
ISC Safety Compliance	Cambodia
UAE RoHS	UAE
NRCS and SABS	South Africa
EAC	Customs Union of the Eurasian Economic Union

License and Warranty

The license and warranty contract ensures your instrument is performing optimally by providing the latest up-to-date hardware and software. The contract guarantees that Oxford Nanopore Technologies support obligations are delivered during the contract period as laid out in sections 4 and 7 of the Nanopore Product Terms and Conditions.

What’s in the box

• MinION Mk1D
• MinION Configuration Test Cell (CTC)
• USB Type-C 0.5 m cable
• Quick Start Guide
• Safety and regulatory documentation

Configuration is the process of testing that communication between the MinION device and the control software on the host computer is operational prior to experimental work being performed. This is carried out in the absence of any chemistry and uses a specific flow cell known as the Configuration Test Cell (CTC).

Product cross-compatibility

The MinION Mk1D can be used together with:

Flow cells

• MinION/GridION Flow Cell R10.4.1 (FLO-MIN114)
• MinION/GridION Flow Cell – RNA (FLO-MIN004RA)
• Flongle Flow Cell (FLO-FLG114)

Kits

• Ligation Sequencing Kit V14 (SQK-LSK114)
• Ligation Sequencing Kit XL V14 (SQK-LSK114-XL)
• Ultra-Long DNA Sequencing Kit V14 (SQK-ULK114)
• Multiplex Ligation Sequencing Kit XL V14 (SQK-MLK114.96-XL)
• Rapid Sequencing Kit V14 (SQK-RAD114)
• Rapid Barcoding Kit 24 V14 (SQK-RBK114.24)
• Rapid Barcoding Kit 96 V14 (SQK-RBK114.96)
• Rapid PCR Barcoding Kit 24 V14 (SQK-RPB114.24)
• Native Barcoding Kit 24 V14 (SQK-NBD114.24)
• Native Barcoding Kit 96 V14 (SQK-NBD114.96)
• 16S Barcoding Kit 24 V14 (SQK-16S114.24)
• cDNA-PCR Sequencing Kit V14 (SQK-PCS114)
• cDNA-PCR Barcoding Kit V14 (SQK-PCB114.24)
• Direct RNA Sequencing Kit (SQK-RNA004)

Software Basecalling:

• MinKNOW
• Dorado

Basecalled reads are available as POD5, FASTQ, and BAM files.

Downstream analysis:

• EPI2ME
• Oxford Nanopore-developed tools and pipelines
• Customer-developed tools and pipelines

Change log

Date	Version	Changes made
6th November 2024	V4	- In 'Technical specifications', the environmental temperature range for the function of electronics was removed from the Table and added below it with an (*) referencing the sequencing temperature range. - In 'License and Warranty', the link for Device Warranty was removed as it is no longer applicable. - In 'What's in the box', the Quick Start Guide, and Safety and regulatory documentation were added. - In 'Product cross-compatibility, under 'Software', availability of basecalled reads as BAM files was added.
31st October 2024	V3	- In 'Shipping and logistics', the ambient temperature range (2-25°C) was changed for clarity to (+2°C to +25°C). - In 'Product cross-compatibility', flow cell (MinION/GridION Flow Cell R9.4.1 (FLO-MIN106D)) and kit (Ligation Sequencing Kit (SQK-LSK110)) compatibilities with legacy chemistry were removed.
25th October 2024	V2	- In 'Product overview', the device description was revised. - In 'Technical specifications', descriptions were revised in the Table to align with other documents and an installation requirement of 5 cm clearance was added. - In 'IT requirements', the importance of the use of USB-C ports only to connect the MinION Mk1D to a computer was added. - A section on 'Declaration of conformity' and 'International standards' were added. - In 'Product cross-compatibility', the list of compatible kits was updated.
2024	V1	Initial document publication