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Rapid metagenomic sequencing for surveillance of bacterial, fungal and viral pathogens using SQK-RPB114.24 (RMS_9215_v114_revF_18Nov2025)

DateVersionLanguageDeviceChanges
11/18/2025RMS_9215_v114_revF_18Nov2025EnglishGridION
18th November 2025 - In section 1, Table under 'Library preparation', the timing for the priming and loading a flow cell was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. - In section 4, 'Reverse transcription and PCR', an important note was added to use the barcode wells only once. - In section 5, 'Clean-up and quantification and adapter attachment' a note was added to check your flow cell at the start of this section. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
11/18/2025RMS_9215_v114_revF_18Nov2025EnglishMinION
18th November 2025 - In section 1, Table under 'Library preparation', the timing for the priming and loading a flow cell was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. - In section 4, 'Reverse transcription and PCR', an important note was added to use the barcode wells only once. - In section 5, 'Clean-up and quantification and adapter attachment' a note was added to check your flow cell at the start of this section. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
11/18/2025RMS_9215_v114_revF_18Nov2025中文GridION
18th November 2025 - In section 1, Table under 'Library preparation', the timing for the priming and loading a flow cell was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. - In section 4, 'Reverse transcription and PCR', an important note was added to use the barcode wells only once. - In section 5, 'Clean-up and quantification and adapter attachment' a note was added to check your flow cell at the start of this section. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
11/18/2025RMS_9215_v114_revF_18Nov2025中文MinION
18th November 2025 - In section 1, Table under 'Library preparation', the timing for the priming and loading a flow cell was increased from 5 min to 10 min. And in 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. - In section 4, 'Reverse transcription and PCR', an important note was added to use the barcode wells only once. - In section 5, 'Clean-up and quantification and adapter attachment' a note was added to check your flow cell at the start of this section. - In section 6, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. The addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
9/2/2025RMS_9215_v114_revE_02Sep2025EnglishGridION
02 September 2025 - Replaced image assets in tables that were missing/bugged and not presenting correctly.
9/2/2025RMS_9215_v114_revE_02Sep2025EnglishMinION
02 September 2025 - Replaced image assets in tables that were missing/bugged and not presenting correctly.
9/2/2025RMS_9215_v114_revE_02Sep2025中文GridION
02 September 2025 - Replaced image assets in tables that were missing/bugged and not presenting correctly.
9/2/2025RMS_9215_v114_revE_02Sep2025中文MinION
02 September 2025 - Replaced image assets in tables that were missing/bugged and not presenting correctly.
9/2/2025RMS_9215_v114_revD_02Sep2025EnglishGridION
02 September 2025 - Replaced image assets in tables that were missing/bugged and not presenting correctly.
9/2/2025RMS_9215_v114_revD_02Sep2025EnglishMinION
02 September 2025 - Replaced image assets in tables that were missing/bugged and not presenting correctly.
9/2/2025RMS_9215_v114_revD_02Sep2025中文GridION
02 September 2025 - Replaced image assets in tables that were missing/bugged and not presenting correctly.
9/2/2025RMS_9215_v114_revD_02Sep2025中文MinION
02 September 2025 - Replaced image assets in tables that were missing/bugged and not presenting correctly.
6/3/2025RMS_9215_v114_revC_03June2025EnglishGridION
03 June 2025 - Added link to the know-how document in the protocol introduction
6/3/2025RMS_9215_v114_revC_03June2025EnglishMinION
03 June 2025 - Added link to the know-how document in the protocol introduction
8/22/2025RMS_9215_v114_revC_03June2025中文GridION
03 June 2025 - Added link to the know-how document in the protocol introduction
8/22/2025RMS_9215_v114_revC_03June2025中文MinION
03 June 2025 - Added link to the know-how document in the protocol introduction
5/22/2025RMS_9215_v114_revB_07May2025EnglishGridION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/22/2025RMS_9215_v114_revB_07May2025EnglishMinION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RMS_9215_v114_revB_07May2025EnglishGridION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
5/7/2025RMS_9215_v114_revB_07May2025EnglishMinION
08th May 2025 - Added information for SQK-RPB114.24 kit format update from vials to plate. - Included instructions for both kit formats: old vial format with Rapid Barcode Primers at 10 μM, new plate format with Rapid Barcode Primers at 1 μM.
4/29/2025RMS_9215_v114_revA_11Dec2024EnglishGridION
Protocol release
4/29/2025RMS_9215_v114_revA_11Dec2024EnglishMinION
Protocol release
1/8/2025RMS_9215_v114_revA_11Dec2024EnglishGridION
Protocol release
1/8/2025RMS_9215_v114_revA_11Dec2024EnglishMinION
Protocol release
12/9/2024VM_RPB_V114_rev15EnglishGridION
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12/9/2024VM_RPB_V114_rev15EnglishMinION
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11/13/2024VM_RPB_V114_rev7EnglishGridION
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11/13/2024VM_RPB_V114_rev7EnglishMinION
-
11/8/2024VM_RPB_V114_rev3EnglishGridION
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11/8/2024VM_RPB_V114_rev3EnglishMinION
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