Ligation sequencing V14 — single-cell transcriptomics with 5' cDNA prepared using 10X Genomics on PromethION (SQK-LSK114) (SST_9204_v114_revJ_25Feb2025)
| Date | Version | Language | Device | Changes | |
|---|---|---|---|---|---|
| 4/9/2025 | SST_9204_v114_revJ_25Feb2025 | English | PromethION | 25th February 2025 In "Overview of the protocol" and "Equipment and consumables", compatibility information has been corrected to say "This protocol is intended for use with the 10X Genomics GEM-X Single Cell 5' Kit (V3) or the Next GEM Single Cell 5' Kit (V2)." | |
| 1/29/2025 | SST_9204_v114_revH_29Jan2025 | English | PromethION | 29th January 2025 In "PCR amplification", the quantification step has been changed to say "Quantify 1 µl of eluted sample using a Qubit fluorometer - recovery aim >100 ng total.". | |
| 11/6/2024 | SST_9204_v114_revG_06Mar2024 | English | PromethION | November 2024 - Formatting fix to custom-ordered oligo sequences table in equipment and consumables to prevent some of the sequence being cut off in the PDF generation. | |
| 8/21/2024 | SST_9204_v114_revF_06Mar2024 | English | PromethION | 21st August 2024 - NEB reagent recommendations for purchase and use updated (Salt T4 ligase in place of Quick Ligation Module). - Updated the Adapter ligation step with new recommended method. | |
| August 21st, 2024 | SST_9204_v114_revE_31Jul2024 | English | - | Download | 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07 May 2024 - Updated library loading recommendation at the end of "Adapter ligation and clean-up" to use 50–100 fmol going into the flow cell. |
| May 14th, 2024 | SST_v9204_v114_revD_06Mar2024 | English | - | Download | 07 May 2024 - Updated library loading recommendation at the end of "Adapter ligation and clean-up" to use 50–100 fmol going into the flow cell. |
| April 12th, 2024 | SST_v9204_v114_revA_06Mar2024 | English | - | Download | - |