30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
LWP_9183_v114_revM_30Jan2025
English
MinION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
LWP_9183_v114_revM_30Jan2025
English
PromethION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/12/2024
LWP_9183_v114_revL_12Dec2024
English
GridION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
LWP_9183_v114_revL_12Dec2024
English
MinION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
LWP_9183_v114_revL_12Dec2024
English
PromethION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/2024
LWP_9183_v114_revK_07Mar2023
English
GridION
21st August 2024
- Updated protocol introduction to new format.
- Updated sample input recommendations to 100 ng for gDNA or amplicons.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024
LWP_9183_v114_revK_07Mar2023
English
MinION
21st August 2024
- Updated protocol introduction to new format.
- Updated sample input recommendations to 100 ng for gDNA or amplicons.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024
LWP_9183_v114_revK_07Mar2023
English
PromethION
21st August 2024
- Updated protocol introduction to new format.
- Updated sample input recommendations to 100 ng for gDNA or amplicons.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated