11th September 2025
- In 'Overview of the protocol', in the Table under 'Library preparation and sequencing', the timing for priming and loading a flow cell was increased from 5 min to 10 min.
- In 'Equipment and consumables', the image of the LSK114 kit in single-use tube format was removed. And instructions for the single-use tube format were removed from section 8, step 2.
- In section 4, 'DNA repair and end-prep', 'Check your flow cell' and section 6, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 4, 'DNA repair and end-prep', step 23, instructions were provided on how to resuspend the AMPure XP bead pellet with nuclease-free water.
- In section 6, 'Priming and loading the flow cell', the instruction for removing the flow cell out of the fridge was moved to the top of this section.
- In 'Troubleshooting', section 11, the video links for priming and loading a MinION Flow Cell were replaced with links for the PromethION Flow Cell.
ONT plc is a member of the producer compliance scheme run by ERP UK Ltd, who manage the submission of documentation in support of WEEE compliance for ONT plc’s manufacture and supply of Electrical and Electronic equipment in the UK.
