Direct RNA sequencing - sequence-specific (SQK-RNA004) (DSS_9197_v4_revC_11Dec2024)
| Date | Version | Language | Device | Changes | |
|---|---|---|---|---|---|
| 12/11/2024 | DSS_9197_v4_revC_11Dec2024 | English | GridION | 11th Dec 2024 - Updated kit content to include information on different RCS vial concentration in new kit batches. - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". | |
| 12/11/2024 | DSS_9197_v4_revC_11Dec2024 | English | MinION | 11th Dec 2024 - Updated kit content to include information on different RCS vial concentration in new kit batches. - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". | |
| 12/11/2024 | DSS_9197_v4_revC_11Dec2024 | English | PromethION | 11th Dec 2024 - Updated kit content to include information on different RCS vial concentration in new kit batches. - Addition of links to MinION Mk1D IT requirements and user manual. - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for EPI2ME in "Downstream analysis". | |
| 7/31/2024 | DSS_9197_v4_revB_20Sep2023 | English | GridION | 31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells. | |
| 7/31/2024 | DSS_9197_v4_revB_20Sep2023 | English | MinION | 31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells. | |
| 7/31/2024 | DSS_9197_v4_revB_20Sep2023 | English | PromethION | 31 July 2024 - Updated the protocol format to the new "slimmed-down" introduction sections. - Updated workflow image in introduction to fix formatting errors in PDF generation - Updated the Reverse transcriptase reagents and steps to the new method using Induro® Reverse Transcriptase (NEB, cat # M0681). - Updated the RNase Inhibitor reagents and steps to the new method using Murine RNase Inhibitor (NEB, M0314). - Updated first Agencourt RNAClean XP bead clean in Library Preparation section to elute into 23 ul Nuclease-free water, retain the full 23 ul and no longer have to add 3 ul of water in the next step. - Updated "Flow cell reuse and returns" section to specify compatibility and use-cases for the Flow Cell Wash Kit with the RNA sequencing kit and RNA flow cells. | |
| July 31st, 2024 | DSS_9197_v4_revA_20Sep2023 | English | Promethion | Download | - |
| July 31st, 2024 | DSS_9197_v4_revA_20Sep2023 | English | Gridion | Download | - |
| July 31st, 2024 | DSS_9197_v4_revA_20Sep2023 | English | Minion | Download | - |