DNA library preparation
Gain a comprehensive insight into DNA
A wide range of library preparation kits is available to suit whole-genome as well as targeted sequencing requirements. Amplification-free kits allow direct sequencing of short to ultra-long fragments of native DNA, eliminating PCR bias and allowing the detection of base modifications alongside standard nucleotides. Amplification-based kits enable whole-genome sequencing from low-input amounts or low-quality DNA (e.g. FFPE).
| Output optimised | Speed optimised | Ultra-long reads optimised | Low input optimised | Targeted sequencing | |
|---|---|---|---|---|---|
| Ligation Sequencing Kit | Rapid Sequencing Kit | Ultra-long DNA Sequencing Kit | Rapid PCR Barcoding Kit | Microbial Amplicon Barcoding Kit | |
| Preparation time | 60 minutes | 10 minutes | 200 mins + 1x O/N incubation | 15 mins + PCR | 60 mins + PCR |
| Input recommendation | ~1000 ng gDNA or 100-200 fmol for amplicons | ~200 ng gDNA or 50 ng for amplicons | 6M cells | 1 - 5 ng gDNA | 10 ng gDNA |
| Fragmentation | Optional | Transposase-based | Transposase-based | Transposase-based | - |
| Amplification | No | No | No | Yes | Yes |
| Barcode options | Native Barcoding Kit 24 Native Barcoding Kit 96 | Rapid Barcoding Kit 24 Rapid Barcoding Kit 96 | - | 24 plex | 24 plex |
| Typical output | High | Medium | Medium | Medium | Medium |
| Adaptive sampling | Yes | Yes | Yes | Yes | - |
| Methylation included | Yes | Yes | Yes | - | - |
Unlock high-accuracy direct sequencing
- Highly accurate detection of genetic variation
- Best-in-class methylation detection
Learn more about adaptive sampling
- Software-controlled target enrichment
- No need for additional library prep
