Precision genomics for hereditary cancer with adaptive sampling
The Oxford Nanopore Hereditary Cancer Panel offers comprehensive germline variant analysis across 258 cancer predisposition genes. The panel uses adaptive sampling: a fast and flexible on-sequencer target enrichment methodology. No lengthy library preparation, baits, or primers required.
Why choose the Oxford Nanopore Hereditary Cancer Panel?
One consolidated assay for deeper genomic and epigenomic insight — in one go.
Rapid, streamlined sequencing — no batching required
Flexible enrichment. Scalable sequencing. Built for every lab.
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PromethION 24
The PromethION 24 (P24) is a high-throughput sequencing device featuring 24 independent flow cells positions, allowing users to sequence multiple samples simultaneously or flexibly scale their experiments.
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What is the Oxford Nanopore Hereditary Cancer Panel?
The Oxford Nanopore Hereditary Cancer Panel (HCP) is a comprehensive sequencing assay targeting 258 full-length genes associated with germline cancer risk. It combines long-read sequencing with Adaptive Sampling for real-time, on-device enrichment, eliminating the need for baits, probes, or primers.
What does the Hereditary Cancer Panel detect?
The panel enables comprehensive genomic and epigenomic profiling, including:
- Single nucleotide variants (SNVs)
- Insertions and deletions (indels)
- Structural variants (SVs) and exon-level deletions
- Complex rearrangements and pseudogenes
- Methylation patterns for epigenetic insights
How many genes are included in the panel?
The HCP includes 258 full-length genes implicated in cancer predisposition, covering exons, introns, and promoters. Key examples: BRCA1, BRCA2, ATM, TP53, PALB2, MSH2, and many more.
How does Adaptive Sampling work on this panel?
Adaptive Sampling leverages Oxford Nanopore’s real-time sequencing to perform software-based targeted sequencing without additional library prep by using digital panels, which contain the target sequences. The digital panel is uploaded to the sequencing software MinKNOW, and during sequencing, DNA molecules containing the target are sequenced, and those without the target are not sequenced based on the first ~400 bp. This enables sequencing enrichment of regions of interest whilst simultaneously generating low-pass whole-genome coverage. By using digital panels rather than baits, probes, or primers, wet-lab workflows are streamlined, and digital panels can be rapidly customised.
What type of sample does the assay require?
The HCP is designed for whole blood DNA input, requiring approximately 1 µg of DNA per sample, with three samples sequenced per PromethION Flow Cell.
How long does it take to complete the workflow?
The end-to-end process from sample extraction to sequencing can be completed within 5 days (≤ 2 hours hands-on time).
How uniform is coverage and enrichment with this panel?
In our HCP application note, we have demonstrated >30x coverage for target regions and ~5x low-pass coverage across the genome can be achieved. The method consistently achieves uniform enrichment and accurately detects both small variants (SNV) and large variants.
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Comprehensive genomic and epigenomic profiling with the Oxford Nanopore Hereditary Cancer Panel
This application note highlights how the HCP provides a scalable, accessible, and cost-effective solution for comprehensive analysis of hereditary cancer genes, with the potential to advance precision oncology.
