Assembling microbial genomes from complex metagenomic samples using long nanopore reads

Overview

Covering a vast array of applications, metagenomic sequencing allows the rapid identification and analysis of culturable microorganisms, and, importantly, has made possible the analysis of those microbes which cannot be cultured. Nanopore sequencing generates any-length reads that span repeat-rich sequences — characteristic of antimicrobial resistance (AMR) genes — and structural variants (SVs), simplifying genome assembly.

This end-to-end workflow provides a simple solution for assembling microbial genomes from metagenomic samples.

In this workflow overview, you will:

• Find out how direct DNA nanopore sequencing enhances assembly of genomes from metagenomic samples
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices