Getting started guide: single-cell transcriptomics

Getting started guide: single-cell transcriptomics

Single-cell RNA sequencing of the human transcriptome is a valuable research tool, shedding light on the complex mechanisms behind developmental processes, immune system functions, and diseases such as cancer. Full-length transcripts can be sequenced in single long nanopore reads, delivering unambiguous detection of isoforms and fusion transcripts that cannot be captured with short-read approaches. Additionally, nanopore reads enable SNV detection across complete transcripts for deep characterisation of clonal evolution and tumour heterogeneity.

This guide introduces the single-cell RNA nanopore sequencing workflow, for ultra-rich data without compromise.

In this getting started guide, you will:

- Find out about the benefits of nanopore sequencing for single-cell transcriptomics, and how it reveals isoform-level information missed by legacy short-read technologies

- Read about the end-to-end single-cell nanopore sequencing workflow

- Discover how to go from 10x Genomics cell-barcoded cDNA samples to sequencing-ready libraries in around three hours

- Find out about the range of high-output PromethION sequencing devices

- Discover how single-cell nanopore sequencing data can be easily analysed using the EPI2ME workflow wf-single-cell

- Read case studies describing how researchers are utilising single-cell nanopore sequencing to enhance research into leukaemia and the mouse retina transcriptome