Same-day whole-plasmid sequencing in your lab

With plasmids being an important tool in molecular biology, verification of the cloned plasmid sequence is essential to confirm no errors are present that could disrupt expression of inserted genes. Despite sequencing being the gold-standard approach for clone verification, traditional technologies presents many limitations, including turnaround time, primer requirements, sequence bias, and sample throughput.

Using nanopore sequencing, researchers can overcome these challenges, with the ability to offer a fast and cost-effective whole-plasmid sequencing workflow that can be easily implemented in your lab.

This talk covers:

• The importance of sequencing whole plasmids – not just inserts
• How to go from purified samples to results in just a few hours
• How nanopore sequencing allows Vertex Pharmaceuticals to save time and effort in their clone verification workflow in AAV research
• How local- or cloud-based analysis keeps your data secure and in house