Improving plant genome assemblies with Pore-C

Overview

Pore-C is an end-to-end workflow combining chromatin conformation capture (3C) with long nanopore sequencing reads, providing contact information that allows existing assemblies to be scaffolded to high contiguity. Pore-C generates higher-order contact information from multiple genomic loci, and because nanopore sequencing is PCR-free, GC-rich and repeat-rich regions can be characterised along with epigenetic modifications.

This workflow is an end-to-end method for the scaffolding of a plant genome assembly to chromosome scale.

In this workflow overview, you will:

• Find out how Pore-C and nanopore sequencing enhances the assembly of plant genomes
• Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
• Learn about our recommended sequencing kit and devices