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Why is a clean-up step required after the DNA repair and end-prep for NBD114.24, but not for NBD114.96?
Why is a clean-up step required after the DNA repair and end-prep for NBD114.24, but not for NBD114.96?
This is due to the different volume of end-prep DNA taken forward between the two different protocols (7.5 µL vs 0.75 µL for NBD114.24 and NBD114.96 respectively). A clean-up is required for NBD114.24 to limit carry over of enzymes from the UltraII and FFPE repair kits.