PromethION 24/48 user manual

DateVersion numberLanguageDeviceChanges
Oct 14 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019Español
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019中文
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019Español
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019中文
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019Español
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019中文
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019Español
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019中文
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019Español
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 14 2024PIC_1013_v1_revZ_12Nov2019中文
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 10 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 10 2024PIC_1013_v1_revZ_12Nov2019Español
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 10 2024PIC_1013_v1_revZ_12Nov2019中文
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 10 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Oct 10 2024PIC_1013_v1_revZ_12Nov2019中文
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Sep 27 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Sep 27 2024PIC_1013_v1_revZ_12Nov2019中文
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Sep 26 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Sep 26 2024PIC_1013_v1_revZ_12Nov2019中文
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
Apr 30 2024PIC_1013_v1_revZ_12Nov2019English
30th April 2024 - Corrected the specifications in "Device information" - "What's in the box" has been updated to include 1x MiniDP/Display port cable, 1x USB-A stick (ISO reset image), and 1x USB-C/USB-A adapter - Added information about the PromethION 24 Combined in "Device information" and "What's in the box", with a disclaimer: "The PromethION 24 Combined must only be unpacked and installed by an Oxford Nanopore Technologies Field Service Engineer."
April 30th, 2024PIC_1013_v1_revY_12Nov2019English Download 20th February 2024 In "Device information", the mains supply voltage and peak power consumption values have been updated. The value for the operating current has been removed.
February 20th, 2024PIC_1013_v1_revX_12Nov2019English Download 18th December 2023 In "Hardware check", the screenshot of the loading bar has been updated.
October 28th, 2023PIC_1013_v1_revV_12Nov2019English Download 9th August 2023 - The device name has been updated from A100 to A-Series - The wording throughout the protocol has been updated for simplicity and clarity
August 9th, 2023PIC_1013_v1_revU_12Nov2019English Download 7th June 2023 In "Downloading the ISO image and burning it to a USB drive", the ISO image download link has been updated.
May 18th, 2023PIC_1013_v1_revT_12Nov2019English Download May 2023 - Updated screenshots with new images from the GUI throughout the document for software release 23.04.X
May 5th, 2023PIC_1013_v1_revS_12Nov2019English Download 5th May 2023 - In "Data management", the command in Step 4 has been corrected.
March 14th, 2023PIC_1013_v1_revR_12Nov2019English Download 13th March 2023 - In "Operating system update" - "If problems are encountered", a note has been added to say "During the process to blank all of the system’s disks, the following screen will be visible showing either ASRock or ASUS with a scrolling circle. This process may take time to complete and be following by a reboot."
March 10th, 2023PIC_1013_v1_revQ_12Nov2019English Download 10th March 2023 - In "Operating system update" - "If problems are encountered", a new FAQ was added about performing a secure wipe. - "Downloading the ISO image and burning it to a USB drive" - the link to the ISO image download has been updated. A comment has been added to the Overview to say "These instructions are for users who did not receive a USB drive with the ISO image. If you have received a USB drive, proceed to the next step: "How to boot from USB"."
March 10th, 2023PIC_1013_v1_revP_12Nov2019English Download 27th February 2023 - In "Data management", erroneous GridION instructions have been changed to those for PromethION.
February 14th, 2023PIC_1013_v1_revO_12Nov2019English Download February 2023 - Updated information in "Installing and powering on the device" for how users log into MinKNOW software using their Community credentials which now requires the use of OKTA as user authentification.
December 21st, 2022PIC_1013_v1_revN_12Nov2019English Download December 2022 - Updated the file name, box link and the SHA256 hash in "Downloading the ISO image and burning it to a USB drive"
September 26th, 2022PIC_1013_v1_revM_12Nov2019English Download 15th September 2022 - "Device information" - added link to the PromethION P24/P48 A100 technical specification document. - "Downloading the ISO image and burning it to a USB drive": the download link and MD5 hash have been updated. The USB drive specification has been changed from 16 GB to 32 GB. - "How to boot from USB": a note has been added that once the rebuild process has been completed and the device has had a power cycle, any previous changes to the device password will have defaulted back to the default password.
August 24th, 2022PIC_1013_v1_revL_12Nov2019English Download 24th August 2022 Updated to reflect main updates for MinKNOW 22.08 release: - 'Updating the software' includes information about update availability and rebooting Other updates: - 'Flow cell check': the instruction for starting the flow cell check has been updated to say "When you see the flow cell type and flow cell IDs recognised, click 'Start' to begin."
August 19th, 2022PIC_1013_v1_revK_12Nov2019English Download 19th August 2022 In "Changing the default password", new instructions have been added for changing the default hostname.
August 10th, 2022PIC_1013_v1_revJ_12Nov2019English Download 14th June 2022 - In "Operating system update on PromethION" - "Completing the restore", a new instruction has been added: "Configure your network".
May 26th, 2022PIC_1013_v1_revI_12Nov2019English Download 26th May 2022 - Updated to direct customers to use the GUI to change your password
May 5th, 2022PIC_1013_v1_revH_12Nov2019English Download 5th May 2022 "Overview" - the technical specification has been updated to include the new PromethION A100 devices. "Installing and powering on the device" - instructions have been updated to include the new PromethION A100 devices. "Data management" - several instruction steps have been updated to include Focal Ubuntu instructions, update installation commands, include steps on how to start/stop the data transfer, and fix issues with code blocks. In "Downloading the ISO image and burning it to a USB drive": - the Rufus link has been corrected to https://rufus.ie/en/ - the ISO image download link has been updated to include file hash information In "Completing the restore", a new instruction has been added: "Eject the bootable USB by going to the File Explorer and selecting the Eject icon next to the USB device."
February 3rd, 2022PIC_1013_v1_revG_12Nov2019English Download 3rd February 2022 New section added: "Operating system update on PromethION"
November 23rd, 2021PIC_1013_v1_revF_12Nov2019English Download 23rd November 2021 - "Hardware check": the section has been updated to include new flow cell behaviour
September 27th, 2021PIC_1013_v1_revE_12Nov2019English Download 27th September 2021 - "Hardware check": the section has been moved under Installation.
July 21st, 2021PIC_1013_v1_revD_12Nov2019English Download 21st July 2021 The document has been renamed to PromethION user manual, with the specific changes below: - The structure of the document has changed to include the sections "Overview", "Installation", "Pre-sequencing checks and sequencing run", "After completing a sequencing run", "Updating the software" and "Troubleshooting" - Hardware check and flow cell check instructions added - New instructions added for data management from the command-line
June 16th, 2021PIC_1013_v1_revC_12Nov2019English Download 16th June 2021 - Updated all screenshots of the MinKNOW GUI to the new 4.3 release - Updated screenshot for loggin on to MinKNOW and sequencing overview on 'Logging on' in the 'Installation' section - Added screenshot of the start page and Hardware check button highlighted and updated the hardware check page in the 'Installation: Running the hardware check' section
January 20th, 2021PIC_1013_v1_revB_12Nov2019English Download 9th July 2020 New UI across all devices Alignment running in MinKNOW Start of security upgrade - Login Custom filtering of reads - q-score and read lengths New barcoding options in the UI Guppy 4.0 including new basecall models Admin interface Update to use Python 3.7 Various improvements to the scripts - new selection criteria at MUX scan, better handling of low-range reads and global flick and drift improvements ont-vbz-hdf-plugin added as part of the release Support for upcoming kits and expansion packs - Ligation Sequencing Kit (SQK-LSK110), Native Barcoding Expansion 1-96 (EXP-NBD196) and Rapid Barcoding Kit SQK-RBK096
December 12th, 2019PIC_1013_v1_revA_12Nov2019English Download