High duplex ligation sequencing DNA V14 (SQK-LSK114)

DateVersionLanguageDeviceChanges
12/12/2024HDL_9190_V114_revI_12Dec2024EnglishGridION
12 December 2024 - Addition of links to MinION Mk1D and GridION IT requirements in "Compatibility of this protocol" and MinION Mk1D user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024HDL_9190_V114_revI_12Dec2024EnglishMinION
12 December 2024 - Addition of links to MinION Mk1D and GridION IT requirements in "Compatibility of this protocol" and MinION Mk1D user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024HDL_9190_V114_revI_12Dec2024EnglishPromethION
12 December 2024 - Addition of links to MinION Mk1D and GridION IT requirements in "Compatibility of this protocol" and MinION Mk1D user manual in "Data acquisition and basecalling", "How to start sequencing". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
7/31/2024HDL_9190_V114_revH_31Jul2024EnglishGridION
31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and T4 Ligase 6th December 2023 - Updated compatibilities to include new expansion FFPPE DNA Repair Buffer v2 (EXP-NEBFFPEV2) - Included updates to the "Data acquisition and high duplex basecalling" to include MinKNOW duplex basecalling with the 5.8 software release - Updated the protocol to the new template format
7/31/2024HDL_9190_V114_revH_31Jul2024EnglishMinION
31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and T4 Ligase 6th December 2023 - Updated compatibilities to include new expansion FFPPE DNA Repair Buffer v2 (EXP-NEBFFPEV2) - Included updates to the "Data acquisition and high duplex basecalling" to include MinKNOW duplex basecalling with the 5.8 software release - Updated the protocol to the new template format
7/31/2024HDL_9190_V114_revH_31Jul2024EnglishPromethION
31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and T4 Ligase 6th December 2023 - Updated compatibilities to include new expansion FFPPE DNA Repair Buffer v2 (EXP-NEBFFPEV2) - Included updates to the "Data acquisition and high duplex basecalling" to include MinKNOW duplex basecalling with the 5.8 software release - Updated the protocol to the new template format
December 6th, 2023HDL_9190_V114_revG_16Jun2023EnglishMinion Download 6th December 2023 - Updated compatibilities to include new expansion FFPPE DNA Repair Buffer v2 (EXP-NEBFFPEV2) - Included updates to the "Data acquisition and high duplex basecalling" to include MinKNOW duplex basecalling with the 5.8 software release - Updated the protocol to the new template format
December 6th, 2023HDL_9190_V114_revG_16Jun2023EnglishPromethion Download 6th December 2023 - Updated compatibilities to include new expansion FFPPE DNA Repair Buffer v2 (EXP-NEBFFPEV2) - Included updates to the "Data acquisition and high duplex basecalling" to include MinKNOW duplex basecalling with the 5.8 software release - Updated the protocol to the new template format
December 6th, 2023HDL_9190_V114_revG_16Jun2023EnglishGridion Download 6th December 2023 - Updated compatibilities to include new expansion FFPPE DNA Repair Buffer v2 (EXP-NEBFFPEV2) - Included updates to the "Data acquisition and high duplex basecalling" to include MinKNOW duplex basecalling with the 5.8 software release - Updated the protocol to the new template format
October 11th, 2023HDL_9190_V114_revE_16Jun2023EnglishMinion Download 11th October 2023 - Updated library prep to include the Salt-T4 DNA Ligase and NEBNext FFPE Repair Buffer v2. - DNA input has been updated in the equipment and consumables section, as well as a new recommendation regarding fragmentation, size selection and SPRI clean steps.
October 11th, 2023HDL_9190_V114_revE_16Jun2023EnglishPromethion Download 11th October 2023 - Updated library prep to include the Salt-T4 DNA Ligase and NEBNext FFPE Repair Buffer v2. - DNA input has been updated in the equipment and consumables section, as well as a new recommendation regarding fragmentation, size selection and SPRI clean steps.
October 11th, 2023HDL_9190_V114_revE_16Jun2023EnglishGridion Download 11th October 2023 - Updated library prep to include the Salt-T4 DNA Ligase and NEBNext FFPE Repair Buffer v2. - DNA input has been updated in the equipment and consumables section, as well as a new recommendation regarding fragmentation, size selection and SPRI clean steps.
September 19th, 2023HDL_9190_V114_revD_16Jun2023EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
September 19th, 2023HDL_9190_V114_revD_16Jun2023EnglishPromethion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
September 19th, 2023HDL_9190_V114_revD_16Jun2023EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation
August 10th, 2023HDL_9190_V114_revC_16Jun2023EnglishMinion Download 10th August 2023 - Clarified input requirements in "Equipment and consumables" section - Removed reference to Guppy basecaller in the "Computer requirements and software"
August 10th, 2023HDL_9190_V114_revC_16Jun2023EnglishPromethion Download 10th August 2023 - Clarified input requirements in "Equipment and consumables" section - Removed reference to Guppy basecaller in the "Computer requirements and software"
August 10th, 2023HDL_9190_V114_revC_16Jun2023EnglishGridion Download 10th August 2023 - Clarified input requirements in "Equipment and consumables" section - Removed reference to Guppy basecaller in the "Computer requirements and software"
August 1st, 2023HDL_9190_V114_revB_16Jun2023EnglishMinion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
August 1st, 2023HDL_9190_V114_revB_16Jun2023EnglishPromethion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
August 1st, 2023HDL_9190_V114_revB_16Jun2023EnglishGridion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
June 29th, 2023HDL_9190_V114_revA_16Jun2023EnglishMinion Download -
June 29th, 2023HDL_9190_V114_revA_16Jun2023EnglishPromethion Download -
June 29th, 2023HDL_9190_V114_revA_16Jun2023EnglishGridion Download -