From portable to high-throughput benchtop devices, real-time nanopore sensing is a new generation of technology uncovering new biology across multiple sectors.
28th January 2025
- In the Flongle branch of "Library preparation", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RSE_9177_v114_revO_09Jan2025
English
GridION
28th January 2025
- In the Flongle branch of "Library preparation", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RSE_9177_v114_revO_09Jan2025
English
MinION
28th January 2025
- In the Flongle branch of "Library preparation", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RSE_9177_v114_revO_09Jan2025
English
PromethION
28th January 2025
- In the Flongle branch of "Library preparation", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RSE_9177_v114_revO_09Jan2025
Español
Flongle
28th January 2025
- In the Flongle branch of "Library preparation", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RSE_9177_v114_revO_09Jan2025
Español
GridION
28th January 2025
- In the Flongle branch of "Library preparation", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RSE_9177_v114_revO_09Jan2025
Español
MinION
28th January 2025
- In the Flongle branch of "Library preparation", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/28/2025
RSE_9177_v114_revO_09Jan2025
Español
PromethION
28th January 2025
- In the Flongle branch of "Library preparation", information about the Flongle Sequencing Expansion has been updated to remove references to the older version of the kit.
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
Flongle
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
GridION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
MinION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
PromethION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
Flongle
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
GridION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
MinION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
PromethION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
Flongle
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
GridION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
MinION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
PromethION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
Flongle
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
GridION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
MinION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
PromethION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
Flongle
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
GridION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
MinION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
English
PromethION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
Flongle
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
GridION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
MinION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
1/9/2025
RSE_9177_v114_revN_09Jan2025
Español
PromethION
9th January 2025
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual alongside MinION Mk1B in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading the MinION and GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during the sequencing run for Kit 14", "Failed to reach target temperature".
11/11/2024
RSE_9177_v114_revM_16Nov2022
English
Flongle
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
11/11/2024
RSE_9177_v114_revM_16Nov2022
English
GridION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
11/11/2024
RSE_9177_v114_revM_16Nov2022
English
MinION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
11/11/2024
RSE_9177_v114_revM_16Nov2022
English
PromethION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
11/11/2024
RSE_9177_v114_revM_16Nov2022
Español
Flongle
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
11/11/2024
RSE_9177_v114_revM_16Nov2022
Español
GridION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
11/11/2024
RSE_9177_v114_revM_16Nov2022
Español
MinION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
11/11/2024
RSE_9177_v114_revM_16Nov2022
Español
PromethION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
3/20/2024
RSE_9177_v114_revM_16Nov2022
English
Flongle
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
3/20/2024
RSE_9177_v114_revM_16Nov2022
English
GridION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
3/20/2024
RSE_9177_v114_revM_16Nov2022
English
MinION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
3/20/2024
RSE_9177_v114_revM_16Nov2022
English
PromethION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
3/20/2024
RSE_9177_v114_revM_16Nov2022
Español
Flongle
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
3/20/2024
RSE_9177_v114_revM_16Nov2022
Español
GridION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
3/20/2024
RSE_9177_v114_revM_16Nov2022
Español
MinION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
3/20/2024
RSE_9177_v114_revM_16Nov2022
Español
PromethION
20th March 2024
- Updated Priming and loading the PromethION Flow Cell page to correct table making up DNA library to loading (no longer use EB as not available in RAD114 kit, now uses Nuclease-free water)
14th September 2023
- Updated the kit contents image in the Equipment and consumables page.
- Flow cell priming mix instructions have been updated to include single-use tube and bottle formats.
14th September 2023
- Updated the kit contents image in the Equipment and consumables page.
- Flow cell priming mix instructions have been updated to include single-use tube and bottle formats.
14th September 2023
- Updated the kit contents image in the Equipment and consumables page.
- Flow cell priming mix instructions have been updated to include single-use tube and bottle formats.
14th September 2023
- Updated the kit contents image in the Equipment and consumables page.
- Flow cell priming mix instructions have been updated to include single-use tube and bottle formats.
06July2023:
- Minor formatting and wording updates to the "Priming and loading the SpotON flow cell" section of the protocol.
- Minor formatting and wording updates to the "Ending the experiment" section of the protocol.
06July2023:
- Minor formatting and wording updates to the "Priming and loading the SpotON flow cell" section of the protocol.
- Minor formatting and wording updates to the "Ending the experiment" section of the protocol.
06July2023:
- Minor formatting and wording updates to the "Priming and loading the SpotON flow cell" section of the protocol.
- Minor formatting and wording updates to the "Ending the experiment" section of the protocol.
06July2023:
- Minor formatting and wording updates to the "Priming and loading the SpotON flow cell" section of the protocol.
- Minor formatting and wording updates to the "Ending the experiment" section of the protocol.